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2015

Identification of Habitat-Specific Biomes of Aquatic Fungal Communities Using a Comprehensive Nearly Full-Length 18S rRNA Dataset Enriched with Contextual Data

Panzer, K. ., Yilmaz, P. ., Weiß, M. ., Reich, L. ., Richter, M. ., Wiese, J. ., … Reich, M. . (2015). Identification of Habitat-Specific Biomes of Aquatic Fungal Communities Using a Comprehensive Nearly Full-Length 18S rRNA Dataset Enriched with Contextual Data. PLoS ONE, 10, e0134377. Abgerufen von http://oceanrep.geomar.de/29516/

Abstract

Molecular diversity surveys have demonstrated that aquatic fungi are highly diverse, and that they play fundamental ecological roles in aquatic systems. Unfortunately, comparative studies of aquatic fungal communities are few and far between, due to the scarcity of adequate datasets. We combined all publicly available fungal 18S ribosomal RNA (rRNA) gene sequences with new sequence data from a marine fungi culture collection. We further enriched this dataset by adding validated contextual data. Specifically, we included data on the habitat type of the samples assigning fungal taxa to ten different habitat categories. This dataset has been created with the intention to serve as a valuable reference dataset for aquatic fungi including a phylogenetic reference tree. The combined data enabled us to infer fungal community patterns in aquatic systems. Pairwise habitat comparisons showed significant phylogenetic differences, indicating that habitat strongly affects fungal community structure. Fungal taxonomic composition differed considerably even on phylum and class level. Freshwater fungal assemblage was most different from all other habitat types and was dominated by basal fungal lineages. For most communities, phylogenetic signals indicated clustering of sequences suggesting that environmental factors were the main drivers of fungal community structure, rather than species competition. Thus, the diversification process of aquatic fungi must be highly clade specific in some cases.The combined data enabled us to infer fungal community patterns in aquatic systems. Pairwise habitat comparisons showed significant phylogenetic differences, indicating that habitat strongly affects fungal community structure. Fungal taxonomic composition differed considerably even on phylum and class level. Freshwater fungal assemblage was most different from all other habitat types and was dominated by basal fungal lineages. For most communities, phylogenetic signals indicated clustering of sequences suggesting that environmental factors were the main drivers of fungal community structure, rather than species competition. Thus, the diversification process of aquatic fungi must be highly clade specific in some cases.
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2015

Journal Article

Katrin Panzer

Pelin Yilmaz

Michael Weiß

Lothar Reich

Michael Richter

Jutta Wiese

Rolf Schmaljohann

Antje Labes

Johannes Imhoff

Frank Glöckner

Marlis Reich

Habitats

Fungal genetics

Phylogenetics

Fungi

Fungal structure

Fresh water

Phylogenetic analysis

Ribosomal RNA
Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae

Wu, B. ., Wiese, J. ., Labes, A. ., Kramer, A. ., Schmaljohann, R. ., & Imhoff, J. F. (2015). Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae. Marine Drugs, 13, 4617–4632. Abgerufen von http://oceanrep.geomar.de/29518/

Abstract

An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC50 value of 5.1 ($\pm$0.2) ?M and 3.2 ($\pm$0.4) \ensuremath\muM, respectively, against methicillin-resistant Staphylococcus aureus.
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2015

Journal Article

Bin Wu

Jutta Wiese

Antje Labes

Annemarie Kramer

Rolf Schmaljohann

Johannes Imhoff

MRSA

antibiotic

lindgomycin

marine fungi

marine natural products
Nature s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

Kunz, A. L., Labes, A. ., Wiese, J. ., Bruhn, T. ., Bringmann, G. ., & Imhoff, J. F. (2015). Nature s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain, Online-Ressource.
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2015

Journal Article

Anna Kunz

Antje Labes

Jutta Wiese

Torsten Bruhn

Gerhard Bringmann

Johannes Imhoff
Antibiotic activity of microbial strains isolated from Mytilus edulis (Kiel Fjord, Baltic Sea)

Motiei, A. ., Wiese, J. ., Wenzel-Storjohann, A. ., Labes, A. ., Imhoff, J. F., & Gorokhova, E. . (2015). Antibiotic activity of microbial strains isolated from Mytilus edulis (Kiel Fjord, Baltic Sea). In Congress of European Microbiologists. Abgerufen von http://oceanrep.geomar.de/30441/
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2015

Conference Paper

A. Motiei

Jutta Wiese

Arlette Wenzel-Storjohann

Antje Labes

Johannes Imhoff

E. Gorokhova

2014

Nature?s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

Kunz, A. L., Labes, A. ., Wiese, J. ., Bruhn, T. ., Bringmann, G. ., & Imhoff, J. F. (2014). Nature?s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain. Marine Drugs, 12, 1699–1714. Abgerufen von http://oceanrep.geomar.de/24372/ (Original work published März 2014)

Abstract

Eight streptophenazines (A?H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I?K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I?K) also showed moderate activities against PDE 4B.
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2014

Journal Article

Anna Kunz

Antje Labes

Jutta Wiese

Torsten Bruhn

Gerhard Bringmann

Johannes Imhoff

marine natural product

phenazine

Baltic Sea

Streptomyces

Halichondria panicea

HPLC-DAD/MS

fermentation

PDE 4

antibiotic
A complete view on the aquatic-derived fungal phylogeny and ecology

Yilmaz, P. ., Glöckner, F. O., Imhoff, J. F., Labes, A. ., Panzer, K. ., Schmaljohann, R. ., … Reich, M. . (2014). A complete view on the aquatic-derived fungal phylogeny and ecology. In 15. International Symposium on Microbiology Ecology. Abgerufen von http://oceanrep.geomar.de/26376/
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2014

Conference Paper

P. Yilmaz

F. Glöckner

Johannes Imhoff

Antje Labes

K. Panzer

Rolf Schmaljohann

M. Weiß

Jutta Wiese

M. Reich

2013

Habitat specific provocation of secondary metabolite production by marine bacteria ? microbial communication and pharmaceutical use

Oesterwalbesloh, J. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2013). Habitat specific provocation of secondary metabolite production by marine bacteria ? microbial communication and pharmaceutical use. In VAAM Jahrestagung 2013. Abgerufen von http://oceanrep.geomar.de/20913/
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2013

Conference Paper

Jan Oesterwalbesloh

Jutta Wiese

Antje Labes

Johannes Imhoff
Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea

Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2013). Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea. European Journal of Phycology, 48, 47–60. Abgerufen von http://oceanrep.geomar.de/19592/

Abstract

Bacteria associated with Fucus vesiculosus and Delesseria sanguinea, two macroalgae from the Kiel Fjord were investigated seasonally over two years by cultivation-based methods. A total of 166 bacterial strains were isolated from the macroalgae, affiliated to seven classes of bacteria (Actinobacteria, Bacilli, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Cytophagia and Flavobacteria). According to 16S rRNA gene sequence similarities they were arranged in 82 phylotypes of\ensuremath>99.0\% sequence identity. Assuming that chemical factors rule the bacteriamacroalga and bacteriabacteria interactions on algal surfaces, we tested the antibiotic activity of the bacterial isolates not only against a panel of four standard test organisms (Bacillus subtilis, Candida glabrata, Escherichia coli and Staphylococcus lentus) but also four macroalga-associated microorganisms: Algicola bacteriolytica and Pseudoalteromonas elyakovii (macroalgal pathogens), and Bacillus algicola and Formosa algae (strains associated with algal surfaces). Organic extracts of more than 51\% of the isolates from the two macroalgae inhibited the growth of at least one of the tested microorganisms. As much as 46\% and 45\% of the isolates derived from F. vesiculosus and D. sanguinea, respectively, showed antimicrobial activity against the set of macroalga-associated bacteria, compared with 13 and 19\% against a standard set of microorganisms. High antibacterial activity against macroalgal pathogens and bacterial competitors support the assumption that complex chemical interactions shape the relationships of bacteria associated with macroalgae and suggest that these bacteria are a rich source of antimicrobial metabolites.
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2013

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff

antimicrobial activities

bacterial cultures

chemical associations

Delesseria

epiphytic bacteria

Fucus

Kiel

macroalgabacteria associations
Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae

Goecke, F. R., Thiel, V. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2013). Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae. Phycologia, 52, 14–24. Abgerufen von http://oceanrep.geomar.de/19593/

Abstract

Bacteria are an inherent part of the biotic environment of algae. Recent investigations revealed that bacterial communities associated with algae were generally highly host specific. Several new bacterial species and genera were isolated from algae, which suggested that algae were an interesting environment for discovery of new bacterial taxa; however, the distribution of the different phylogenetic groups among those isolates remained unclear, and this information could help to explain specific associations. We conducted a phylogenetic study based on 16S rRNA gene sequences available in GenBank, including 101 validly described bacterial species that were isolated from eukaryotic macro- and micro-algae from marine and freshwater environments. These species were distributed among six bacterial phyla, including: Bacteroidetes (42 species), Proteobacteria (36 species), and Firmicutes, Actinobacteria, Verrucomicrobia and Planctomycetes (23 species). Bacterial species and strains that carried out similar metabolic functions were found to colonize similar algal taxa or algal groups. This assumption was supported by information available from bacterial species descriptions: (1) Most of the bacteria described from microalgae grouped into the Roseobacter clade (Alphaproteobacteria), which indicated that members of this group were well adapted for life in close association with phytoplankton; and (2) 32\% of the bacterial species, mainly isolates from macroalgae, were able to decompose macroalgal polysaccharides. Because algal-bacterial association are still under-studied in various algal groups, we expect a great number of new bacterial taxa to be discovered in the future.
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2013

Journal Article

Franz Goecke

Vera Thiel

Jutta Wiese

Antje Labes

Johannes Imhoff

Enzyme

Macroalgae

Microbe-alga

Phytoplankton

DINOFLAGELLATE GYMNODINIUM-CATENATUM

MARINE ROSEOBACTER LINEAGE

DISSOLVED ORGANIC-MATTER

IN-SITU HYBRIDIZATION

EPIPHYTIC BACTERIAL

LAMINARIA-JAPONICA

GEN. NOV.

COMMUNITY COMPOSITION

ALEXANDRIUM SPP.

BROWN ALGA

2012

Beneficial effects of 2,4-diacetylphloroglucinol- producing pseudomonads on the marine alga Saccharina latissima

Nagel, K. ., Schneemann, I. ., Kajahn, I. ., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Beneficial effects of 2,4-diacetylphloroglucinol- producing pseudomonads on the marine alga Saccharina latissima. Aquatic Microbial Ecology, 67, 239–249. Abgerufen von http://oceanrep.geomar.de/19108/ (Original work published 2025)

Abstract

Pseudomonas strains were shown to be regularly associated with the brown macroalga Saccharina latissima from the Baltic Sea, studied over several years, and were identified as producers of the antimicrobially active compound 2,4-diacetylphloroglucinol. These findings support the assumption of a stable association between the Pseudomonas spp. strains and S. latissima in the Baltic Sea. The metabolite profile of the Pseudomonas spp. comprised monoacetylphloroglucinol, 2,4-diacetylphloroglucinol, pyoluteorin and several rhizoxins, which exhibited broad-spectrum antibiotic activities against Gram-positive and Gram-negative bacteria as well as against fungi. Because the antibiotic activities included the inhibition of the 2 algal pathogens Pseudoalteromonas elyakovii and Algicola bacteriolytica, we propose a beneficial effect of these marine pseudomonads on their host S. latissima.
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2012

Journal Article

Kerstin Nagel

Imke Schneemann

Inga Kajahn

Antje Labes

Jutta Wiese

Johannes Imhoff

Pseudomonas protegens ? Alga?bacteria association ? 2

4-diacetylphloroglucinol ? Pyoluteorin ? Rhizoxin ? Brown macroalgae

RESISTANT STAPHYLOCOCCUS-AUREUS

FLUORESCENT PSEUDOMONAS

LAMINARIA-SACCHARINA

SECONDARY METABOLITES

IN-VITRO

BACTERIA

PF-5

JAPONICA

STRAINS

AGENT

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