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2013

Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae

Goecke, F. R., Thiel, V. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2013). Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae. Phycologia, 52, 14–24. Abgerufen von http://oceanrep.geomar.de/19593/

Abstract

Bacteria are an inherent part of the biotic environment of algae. Recent investigations revealed that bacterial communities associated with algae were generally highly host specific. Several new bacterial species and genera were isolated from algae, which suggested that algae were an interesting environment for discovery of new bacterial taxa; however, the distribution of the different phylogenetic groups among those isolates remained unclear, and this information could help to explain specific associations. We conducted a phylogenetic study based on 16S rRNA gene sequences available in GenBank, including 101 validly described bacterial species that were isolated from eukaryotic macro- and micro-algae from marine and freshwater environments. These species were distributed among six bacterial phyla, including: Bacteroidetes (42 species), Proteobacteria (36 species), and Firmicutes, Actinobacteria, Verrucomicrobia and Planctomycetes (23 species). Bacterial species and strains that carried out similar metabolic functions were found to colonize similar algal taxa or algal groups. This assumption was supported by information available from bacterial species descriptions: (1) Most of the bacteria described from microalgae grouped into the Roseobacter clade (Alphaproteobacteria), which indicated that members of this group were well adapted for life in close association with phytoplankton; and (2) 32\% of the bacterial species, mainly isolates from macroalgae, were able to decompose macroalgal polysaccharides. Because algal-bacterial association are still under-studied in various algal groups, we expect a great number of new bacterial taxa to be discovered in the future.
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2013

Journal Article

Franz Goecke

Vera Thiel

Jutta Wiese

Antje Labes

Johannes Imhoff

Enzyme

Macroalgae

Microbe-alga

Phytoplankton

DINOFLAGELLATE GYMNODINIUM-CATENATUM

MARINE ROSEOBACTER LINEAGE

DISSOLVED ORGANIC-MATTER

IN-SITU HYBRIDIZATION

EPIPHYTIC BACTERIAL

LAMINARIA-JAPONICA

GEN. NOV.

COMMUNITY COMPOSITION

ALEXANDRIUM SPP.

BROWN ALGA
Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea

Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2013). Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea. European Journal of Phycology, 48, 47–60. Abgerufen von http://oceanrep.geomar.de/19592/

Abstract

Bacteria associated with Fucus vesiculosus and Delesseria sanguinea, two macroalgae from the Kiel Fjord were investigated seasonally over two years by cultivation-based methods. A total of 166 bacterial strains were isolated from the macroalgae, affiliated to seven classes of bacteria (Actinobacteria, Bacilli, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Cytophagia and Flavobacteria). According to 16S rRNA gene sequence similarities they were arranged in 82 phylotypes of\ensuremath>99.0\% sequence identity. Assuming that chemical factors rule the bacteriamacroalga and bacteriabacteria interactions on algal surfaces, we tested the antibiotic activity of the bacterial isolates not only against a panel of four standard test organisms (Bacillus subtilis, Candida glabrata, Escherichia coli and Staphylococcus lentus) but also four macroalga-associated microorganisms: Algicola bacteriolytica and Pseudoalteromonas elyakovii (macroalgal pathogens), and Bacillus algicola and Formosa algae (strains associated with algal surfaces). Organic extracts of more than 51\% of the isolates from the two macroalgae inhibited the growth of at least one of the tested microorganisms. As much as 46\% and 45\% of the isolates derived from F. vesiculosus and D. sanguinea, respectively, showed antimicrobial activity against the set of macroalga-associated bacteria, compared with 13 and 19\% against a standard set of microorganisms. High antibacterial activity against macroalgal pathogens and bacterial competitors support the assumption that complex chemical interactions shape the relationships of bacteria associated with macroalgae and suggest that these bacteria are a rich source of antimicrobial metabolites.
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2013

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff

antimicrobial activities

bacterial cultures

chemical associations

Delesseria

epiphytic bacteria

Fucus

Kiel

macroalgabacteria associations

2012

The Second Skin: Ecological Role of Epibiotic Biofilms on Marine Organisms

Wahl, M. ., Goecke, F. R., Labes, A. ., Dobretsov, S. ., & Weinberger, F. . (2012). The Second Skin: Ecological Role of Epibiotic Biofilms on Marine Organisms. Frontiers in Microbiology, 3, 292. Abgerufen von http://oceanrep.geomar.de/15124/

Abstract

In the aquatic environment, biofilms on solid surfaces are omnipresent. The outer body surface of marine organisms often represents a highly active interface between host and biofilm. Since biofilms on living surfaces have the capacity to affect the fluxes of information, energy, and matter across the host?s body surface, they have an important ecological potential to modulate the abiotic and biotic interactions of the host. Here we review existing evidence how marine epibiotic biofilms affect their hosts? ecology by altering the properties of and processes across its outer surfaces. Biofilms have a huge potential to reduce its host?s access to light, gases, and/or nutrients and modulate the host?s interaction with further foulers, consumers, or pathogens. These effects of epibiotic biofilms may intensely interact with environmental conditions. The quality of a biofilm?s impact on the host may vary from detrimental to beneficial according to the identity of the epibiotic partners, the type of interaction considered, and prevailing environmental conditions. The review concludes with some unresolved but important questions and future perspectives.
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2012

Journal Article

Martin Wahl

Franz Goecke

Antje Labes

Sergey Dobretsov

Florian Weinberger

Stress

microbe-macroorganism interaction

modulation of interactions

epibiosis

chemical ecology

biofilm
Bacteria associations with co-occurring macroalgae: Host, epiphyte and environmental factors

Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Bacteria associations with co-occurring macroalgae: Host, epiphyte and environmental factors. European Journal of Phycology, 46, 45–50. Abgerufen von http://oceanrep.geomar.de/19586/

Abstract

Bacteria associated with 2 macroalgae growing in the Kiel Fjord (Baltic Sea) were investigated seasonally over two years by scanning electron microscopy and cultivation methods. Seasonal variations and significant differences between both macroalgal species with respect to the associated bacteria were observed. 166 bacterial strains were isolated from the macroalgae and classified by phylogenetic analysis of 16S rRNA gene sequences. The strains formed 82 phylotypes according to sequence similarities of499.0\%. Samples from Fucus vesiculosus revealed 43 and from Delesseria sanguinea 57 bacterial phylotypes. They affiliated to Actinobacteria, Alphaproteobacteria, Bacilli, Betaproteobacteria, Flavobacteria, Gammaproteobacteria, and Sphingobacteria. Following the assumption that chemical interactions rule the bacteria-macroalga associations, we tested the biological activity of both macroalgae and isolated bacterial strains against a panel comprising standard and ecologically relevant microorganisms (Gram-positive and Gram-negative bacteria, including macroalgal pathogens and surface associated strains, and a yeast). Both macroalgae presented inhibitory activity against at least one microorganism, but overall the inhibitory activities were low. In contrast, all extracts stimulated growth of many of the tested bacteria. While growth stimulation was common concerning bacteria associated with macroalgae, no stimulation was shown for the standard set of bacteria (with exception of one case of stimulation of the plant pathogen Erwinia amylovora). Regarding the potential biological activity of microorganisms associated with macroalgae, more than 60\% of the bacterial phylotypes inhibited the growth of at least one microorganism. A higher proportion of strains showed antimicrobial activity against the set of bacteria associated with macroalgae as compared to standard set of microorganisms. Certain phylotypes are consistently found as epiphytes, suggesting their specific association with macroalgae. The presented positive and negative effects of macroalgal extracts on growth of macroalga-associated bacteria, significant bacterial activity against macroalgal pathogens and competitors, and potential degradative capabilities support the assumption that complex chemical interactions shape the bacteria-macroalga relationships.
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2012

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff
Differences in bacterial association with macroalgae are product of environmental and chemical interactions = Diferencias de asociaci\ on bacteriana en macroalgas son producto de interacciones qu\ imicas y ambientales

Goecke, F. R., Staufenberger, T. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2012). Differences in bacterial association with macroalgae are product of environmental and chemical interactions = Diferencias de asociaci\ on bacteriana en macroalgas son producto de interacciones qu\ imicas y ambientales. In 3. Latin-American Congress of Algal Biotechnology. Abgerufen von http://oceanrep.geomar.de/19541/
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2012

Conference Paper

Franz Goecke

Tim Staufenberger

Jutta Wiese

Antje Labes

Johannes Imhoff
Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea

Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea. Revista De Biologia Marina Y Oceanografia, 47, 75–86. Abgerufen von http://oceanrep.geomar.de/14347/

Abstract

It is assumed that the biological characteristics of the bacterial strains used in bioactivity tests have strong influences on their susceptibility against antibacterial compounds. Therefore, the selection of bacterial test strains may rush conclusions on the effect of macroalgal extracts and metabolites on bacteria. To proof this assumption, we have analysed the biological activities of crude extracts of 16 macroalgae from the coastal waters of Kiel Fjord (Germany), and tested their effect against a panel of 10 microorganisms comprising 5 standard test strains of bacteria and 5 macroalga-associated bacteria. Fourteen macroalgae (88\%) displayed antibacterial activity against at least one of the test strains. Despite the high proportion of extracts exhibiting antimicrobial activity, only 3 strains of the standard set were susceptible to macroalgal extracts and the overall activities were low (less than 80\% of inhibition). Most of active extracts inhibited Bacillus subtilis, while no inhibition effects were found against Erwinia amylovora, Escherichia coli, and the macroalga-associated bacteria. In contrast, all extracts produced stimulatory growth effects of at least two of the tested bacteria. While growth stimulation of standard set of bacteria was rare (22.5\% of total tests) with exception of plant pathogen Erwinia amylovora and two cases of Staphylococcus lentus, it was common among bacteria associated with macroalgae (77.5\%), especially Bacillus algicola, Pseudomonas marincola and both algal-pathogenic bacteria. This study demonstrates that macroalgal extracts can display different effects, i.e., inhibition or stimulation of bacterial growth depending on the origin of the test strains, which are derived from a standard panel or from the marine environment, respectively.
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2012

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff

Microbiology

Marine algae

antibacterial activities

stimulation of bacterial growth

Kiel fjord
Observation of bacteria over the surface of released oogonia from Fucus vesiculosus L (Phaeophyceae)

Goecke, F. R., Labes, A. ., Wiese, J. ., Schmaljohann, R. ., & Imhoff, J. F. (2012). Observation of bacteria over the surface of released oogonia from Fucus vesiculosus L (Phaeophyceae). Gayana Botanica, 69, 376–379. Abgerufen von http://oceanrep.geomar.de/19591/
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2012

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Rolf Schmaljohann

Johannes Imhoff
Phylogenetic relationships among bacteria described from algae: Algae are a distinct source of new taxa

Goecke, F. R., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2012). Phylogenetic relationships among bacteria described from algae: Algae are a distinct source of new taxa. In 3. Latin-American Congress of Algal Biotechnology. Abgerufen von http://oceanrep.geomar.de/19547/
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2012

Conference Paper

Franz Goecke

Jutta Wiese

Antje Labes

Johannes Imhoff
Phylogenetic relationships among bacteria described from algae: Distinct source of new taxa

Goecke, F. R., Thiel, V. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2012). Phylogenetic relationships among bacteria described from algae: Distinct source of new taxa. In Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie. Abgerufen von http://oceanrep.geomar.de/19548/
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2012

Conference Paper

Franz Goecke

Vera Thiel

Jutta Wiese

Antje Labes

Johannes Imhoff
A Novel Phytomyxean Parasite Associated with Galls on the Bull-Kelp Durvillaea antarctica (Chamisso) Hariot

Goecke, F. R., Wiese, J. ., Nunez, A. ., Labes, A. ., Imhoff, J. F., & Neuhauser, S. . (2012). A Novel Phytomyxean Parasite Associated with Galls on the Bull-Kelp Durvillaea antarctica (Chamisso) Hariot. PLoS ONE, 7, e45358. Abgerufen von http://oceanrep.geomar.de/19148/

Abstract

Durvillaea antarctica (Fucales, Phaeophyceae) is a large kelp of high ecological and economic significance in the Southern Hemisphere. In natural beds along the central coast of Chile (Pacific Ocean), abnormal growth characterized by evident gall development and discolorations of the fronds/thallus was observed. Analysing these galls by light microscopy and scanning electron microscopy revealed the presence of endophytic eukaryotes showing typical characteristics for phytomyxean parasites. The parasite developed within enlarged cells of the subcortical tissue of the host. Multinucleate plasmodia developed into many, single resting spores. The affiliation of this parasite to the Phytomyxea (Rhizaria) was supported by 18S rDNA data, placing it within the Phagomyxida. Similar microorganisms were already reported once 23 years ago, indicating that these parasites are persistent and widespread in D. antarctica beds for long times. The symptoms caused by this parasite are discussed along with the ecological and economic consequences. Phytomyxean parasites may play an important role in the marine ecosystem, but they remain understudied in this environment. Our results demonstrate for the first time the presence of resting spores in Phagomyxida, an order in which resting spores were thought to be absent making this the first record of a phagomyxean parasite with a complete life cycle so far, challenging the existing taxonomic concepts within the Phytomyxea. The importance of the here described resting spores for the survival and ecology of the phagomyxid parasite will be discussed together with the impact this parasite may have on the strongest seaweed of the world , which is an important habitat forming and economic resource from the Southern Hemisphere.
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2012

Journal Article

Franz Goecke

Jutta Wiese

Alejandra Nunez

Antje Labes

Johannes Imhoff

Sigrid Neuhauser

SP-NOV

LESSONIA-NIGRESCENS

MARINE MACROALGAE

CENTRAL CHILE

NEW-ZEALAND

PHAEOPHYTA

RHODOPHYTA

ALGAE

DURVILLEALES

INFECTIONS

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2013

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