Bin Ali, N. ., Petersen, K. ., & Mäntylä, M. . , V. (2012). Testing highly complex system of systems: an industrial case study. In Proceedings of the 2012 ACM-IEEE International Symposium on Empirical Software Engineering and Measurement (S. 211–220).
Silber, J. ., Ohlendorf, B. ., Erhard, A. ., Labes, A. ., & Imhoff, J. F. (2012). Marine fungi: a rich source for natural products. In SUBMARINER Blue Biotechnology Cooperation Event: New Strategies and Future Perspectives. Abgerufen von http://oceanrep.geomar.de/20272/
Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea. Revista De Biologia Marina Y Oceanografia, 47, 75–86. Abgerufen von http://oceanrep.geomar.de/14347/
Abstract
It is assumed that the biological characteristics of the bacterial strains used in bioactivity tests have strong influences on their susceptibility against antibacterial compounds. Therefore, the selection of bacterial test strains may rush conclusions on the effect of macroalgal extracts and metabolites on bacteria. To proof this assumption, we have analysed the biological activities of crude extracts of 16 macroalgae from the coastal waters of Kiel Fjord (Germany), and tested their effect against a panel of 10 microorganisms comprising 5 standard test strains of bacteria and 5 macroalga-associated bacteria. Fourteen macroalgae (88\%) displayed antibacterial activity against at least one of the test strains. Despite the high proportion of extracts exhibiting antimicrobial activity, only 3 strains of the standard set were susceptible to macroalgal extracts and the overall activities were low (less than 80\% of inhibition). Most of active extracts inhibited Bacillus subtilis, while no inhibition effects were found against Erwinia amylovora, Escherichia coli, and the macroalga-associated bacteria. In contrast, all extracts produced stimulatory growth effects of at least two of the tested bacteria. While growth stimulation of standard set of bacteria was rare (22.5\% of total tests) with exception of plant pathogen Erwinia amylovora and two cases of Staphylococcus lentus, it was common among bacteria associated with macroalgae (77.5\%), especially Bacillus algicola, Pseudomonas marincola and both algal-pathogenic bacteria. This study demonstrates that macroalgal extracts can display different effects, i.e., inhibition or stimulation of bacterial growth depending on the origin of the test strains, which are derived from a standard panel or from the marine environment, respectively.
Podtschaske, B. ., & Friesdorf, W. . (2012). Sicherheit fördern durch interprofessionelle Kooperation. Mt -Medizintechnik. Organ Des Fachverbandes Biomedizinische Technik e.V. (fbmt), Des Bundesverbands Der Sachverständigen für Medizinprodukte BSM Sowie Der VDI-Gesellschaft Technologies of Life Sciences, Fachbereich Medizintechnik., 132(4), 143–145. Abgerufen von https://www.mt-medizintechnik.de/zeitschrift-mt-medizintechnik/
Bin Ali, N. ., & Petersen, K. . (2012). A consolidated process for software process simulation: State of the art and industry experience. In 2012 38th Euromicro Conference on Software Engineering and Advanced Applications (S. 327–336).
Labes, A. . (2012). Meer Kosmetik. Neue Rohstoffe und Kosmetikkonzepte aus dem Meer ? was können marine mikrobielle Ressourcen dazu beitragen?. In L Oreal Pressekonferenz. Abgerufen von http://oceanrep.geomar.de/15352/
Staufenberger, T. ., Imhoff, J. F., & Labes, A. . (2012). First crenarchaeal chitinase found in Sulfolobus tokodaii. Microbiological Research, 167, 262–269. Abgerufen von http://oceanrep.geomar.de/12897/
Abstract
This is the first description of a functional chitinase gene within the crenarchaeotes. Here we report of the heterologues expression of the ORF BAB65950 from Sulfolobus tokodaii in E. coli. The resulting protein degraded chitin and was hence classified as chitinase (EC 3.2.4.14). The protein characterization revealed a specific activity of 75 mU/mg using colloidal chitin as substrate. The optimal activity of the enzyme was measured at pH 2.5 and 70 ?C, respectively. A dimeric enzyme configuration is proposed. According to amino acid sequence similarities chitinases are attributed to the two glycoside hydrolase families 18 and 19. The derived amino acid sequence of the S. tokodaii gene differed from sequences of these two glycoside hydrolase families. However, within a phylogenetic tree of protein sequences, the crenarchaeal sequence of S. tokodaii clustered in close proximity to members of the glycoside hydrolase family 18.
Tuschy, I. ., Render, M. ., & Vogelsang, A. . (2012). Planung, Einsatz und Entwicklung thermischer Kraftwerke mittlerer Größe unter neuen energiewirtschaftlichen Rahmenbedingungen. In M. . Beckmann & A. . Hurtado (Hrsg.), Kraftwerkstechnik (Bd. 4). Neuruppin: TK-Verlag.