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2016

From Discovery to Production: Biotechnology of Marine Fungi for the Production of New Antibiotics

Silber, J., Kramer, A., Labes, A., & Tasdemir, D. (2016). From Discovery to Production: Biotechnology of Marine Fungi for the Production of New Antibiotics. Marine Drugs, 14, 137. http://doi.org/10.3390/md14070137 (Original work published jul)
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2016

Journal Article

Johanna Silber

Annemarie Kramer

Antje Labes

Deniz Tasdemir

2014

Malettinin E, an antibacterial and antifungal tropolone produced by a marine Cladosporium strain

Silber, J., Ohlendorf, B., Labes, A., Wenzel-Storjohann, A., Näther, C., & Imhoff, J. F. (2014). Malettinin E, an antibacterial and antifungal tropolone produced by a marine Cladosporium strain. Frontiers In Marine Science, 1, 1-6. Abgerufen von http://oceanrep.geomar.de/25470/ (Original work published August)

Abstract

The isolation and structure elucidation of malettinins A?C (1?3) along with the new malettinin E (4) are described. The compounds were produced by the fungus Cladosporium sp. strain KF501, which was isolated from the German Wadden Sea. The malettinins are built up of tropolone/dihydropyran ring structures linked to a furan ring. The structure elucidation of the isolated compounds was achieved by means of one- and two-dimensional NMR spectroscopy supported by mass and UV data. The relative configuration of 4 was determined on the basis of single-crystal X-ray diffraction analysis. 1?4 exhibited antibacterial and antifungal activities when profiled against Xanthomonas campestris and Trichophyton rubrum. The influence of the chemical structure of the furan ring and of configurational changes on biological activities was observed.
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2014

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Arlette Wenzel-Storjohann

Christian Näther

Johannes Imhoff

marinenaturalproducts

fungalnaturalproducts

antibacterial

antifungal

Cladosporium sp.

malettinin

tropolone
A Phenotypic Screening Approach to Identify Anticancer Compounds Derived from Marine Fungi

Ellinger, B., Silber, J., Prashar, A., Landskron, J., Weber, J., Rehermann, S., u. a. (2014). A Phenotypic Screening Approach to Identify Anticancer Compounds Derived from Marine Fungi. Assay And Drug Development Technologies, 12, 162-175. Abgerufen von http://oceanrep.geomar.de/24658/ (Original work published April)

Abstract

This study covers the isolation, testing, and identification of natural products with anticancer properties. Secondary metabolites were isolated from fungal strains originating from a variety of marine habitats. Strain culture protocols were optimized with respect to growth media composition and fermentation conditions. From these producers, isolated compounds were screened for their effect on the viability and proliferation of a subset of the NCI60 panel of cancer cell lines. Active compounds of interest were identified and selected for detailed assessments and structural elucidation using nuclear magnetic resonance. This revealed the majority of fungal-derived compounds represented known anticancer chemotypes, confirming the integrity of the process and the ability to identify suitable compounds. Examination of effects of selected compounds on cancer-associated cell signaling pathways used phospho flow cytometry in combination with 3D fluorescent cell barcoding. In parallel, the study addressed the logistical aspects of maintaining multiple cancer cell lines in culture simultaneously. A potential solution involving microbead-based cell culture was investigated (BioLevitator, Hamilton). Selected cell lines were cultured in microbead and 2D methods and cell viability tests showed comparable compound inhibition in both methods (R-2=0.95). In a further technology assessment, an image-based assay system was investigated for its utility as a possible complement to ATP-based detection for quantifying cell growth and viability in a label-free manner.
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2014

Journal Article

Bernhard Ellinger

Johanna Silber

Anjali Prashar

Johannes Landskron

Jonas Weber

Sarah Rehermann

Franz-Josef Müller

Stephen Smith

Stephen Wrigley

Kjetil en

Philip Gribbon

Antje Labes

Johannes Imhoff

marine fungi

natural products

cancer

pharmacology

leukemia
A phenotypic screening approach to identify anti-cancer compounds derived from marine fungi

Ellinger, B., Landskron, J., Prashar, A., Silber, J., Wrigley, S., Tasken, K., u. a. (2014). A phenotypic screening approach to identify anti-cancer compounds derived from marine fungi. In ELRIG 2014 Research and Innovation - Impact into Cancer. Abgerufen von http://oceanrep.geomar.de/25550/
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2014

Conference Paper

B. Ellinger

J. Landskron

A. Prashar

Johanna Silber

S. Wrigley

K. Tasken

Antje Labes

P. Gribbon
Bioactive metabolites of a marine Calcarisporium sp.

Silber, J., Ohlendorf, B., Erhard, A., Labes, A., & Imhoff, J. F. (2014). Bioactive metabolites of a marine Calcarisporium sp. In ELRIG 2014 Research and Innovation - Impact into Cancer. Abgerufen von http://oceanrep.geomar.de/25551/
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2014

Conference Paper

Johanna Silber

Birgit Ohlendorf

Arlette Erhard

Antje Labes

Johannes Imhoff
Establishing the Secondary Metabolite Profile of a Marine Fungus: Tolypocladium geodes MF458

Kebede, B., Wrigley, S., Prashar, A., Rahlff, J., Ellinger, B., Silber, J., & Labes, A. (2014). Establishing the Secondary Metabolite Profile of a Marine Fungus: Tolypocladium geodes MF458. In ELRIG 2014 Research and Innovation - Impact into Cancer. Abgerufen von http://oceanrep.geomar.de/25552/
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2014

Conference Paper

Bethlehem Kebede

Stephen Wrigley

Anjali Prashar

Janina Rahlff

Bernhard Ellinger

Johanna Silber

Antje Labes

2013

Calcaripeptides A?C, Cyclodepsipeptides from a Calcarisporium Strain

Silber, J., Ohlendorf, B., Labes, A., Näther, C., & Imhoff, J. F. (2013). Calcaripeptides A?C, Cyclodepsipeptides from a Calcarisporium Strain. Journal Of Natural Products, 76, 1461-1467. Abgerufen von http://oceanrep.geomar.de/21997/ (Original work published Juli)

Abstract

The isolation and structure elucidation of the novel calcaripeptides A (1), B (2), and C (3) and studies on their biosynthetic origin are described. The calcaripeptides were identified from Calcarisporium sp. strain KF525, which was isolated from the German Wadden Sea. Compounds 1?3 are macrocyclic structures composed of a proline and a phenylalanine residue as well as a nonpeptidic substructure. Structure elucidation was achieved by applying one- and two-dimensional NMR spectroscopy supported by high-resolution mass spectrometry. X-ray crystallography was performed to determine the relative configuration of 1. The absolute configuration of 1 was assigned by HPLC of the amino acids after hydrolysis of the molecule and derivatization with chiral agents. Studies on the biosynthesis by feeding 13C-labeled substrates revealed that the nonpeptidic part of 1 originates from acetate and l-methionine. The involvement of a hybrid between a polyketide synthase and a nonribosomal peptide synthetase in the biosynthesis of the calcaripeptides is discussed.
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2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Christian Näther

Johannes Imhoff
Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain

Silber, J., Ohlendorf, B., Labes, A., Erhard, A., & Imhoff, J. F. (2013). Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain. Marine Drugs, 11, 3309-3323. Abgerufen von http://oceanrep.geomar.de/21998/ (Original work published August)

Abstract

Bioactive compounds were detected in crude extracts of the fungus, Calcarisporium sp. KF525, which was isolated from German Wadden Sea water samples. Purification of the metabolites from the extracts yielded the five known polyesters, 15G256\ensuremath\alpha, \ensuremath\alpha-2, \ensuremath\beta, \ensuremath\beta-2 and \ensuremath\pi (1?5), and five new derivatives thereof, named calcarides A?E (6?10). The chemical structures of the isolated compounds were elucidated on the basis of one- and two-dimensional NMR spectroscopy supported by UV and HRESIMS data. The compounds exhibited inhibitory activities against Staphylococcus epidermidis, Xanthomonas campestris and Propionibacterium acnes. As the antibacterial activities were highly specific with regard to compound and test strain, a tight structure-activity relationship is assumed.
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2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Arlette Erhard

Johannes Imhoff

fungal natural products

Calcarisporium sp.

calcarides A?E

15G256 components

macrocyclic and linear polyesters

antibacterial activities

antibiotic

marine natural products

2012

Marine fungi: a rich source for natural products

Silber, J., Ohlendorf, B., Erhard, A., Labes, A., & Imhoff, J. F. (2012). Marine fungi: a rich source for natural products. In SUBMARINER Blue Biotechnology Cooperation Event: New Strategies and Future Perspectives. Abgerufen von http://oceanrep.geomar.de/20272/
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2012

Conference Paper

Johanna Silber

Birgit Ohlendorf

Arlette Erhard

Antje Labes

Johannes Imhoff
Secondary metabolites of fungi from the German Wadden Sea

Silber, J., Ohlendorf, B., Erhard, A., Labes, A., Goodfellow, M., & Imhoff, J. F. (2012). Secondary metabolites of fungi from the German Wadden Sea. In Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie. Abgerufen von http://oceanrep.geomar.de/19556/
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2012

Conference Paper

Johanna Silber

Birgit Ohlendorf

Arlette Erhard

Antje Labes

M. Goodfellow

Johannes Imhoff

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