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2017

Establishing the Secondary Metabolite Profile of the Marine Fungus: Tolypocladium geodes sp. MF458 and Subsequent Optimisation of Bioactive Secondary Metabolite Production

Kebede, B. ., Wrigley, S. ., Prashar, A. ., Rahlff, J. ., Wolf, M. ., Reinshagen, J. ., … Ellinger, B. . (2017). Establishing the Secondary Metabolite Profile of the Marine Fungus: Tolypocladium geodes sp. MF458 and Subsequent Optimisation of Bioactive Secondary Metabolite Production. Marine Drugs, 15, 84.
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2017

Journal Article

B. Kebede

S.K. Wrigley

A. Prashar

J. Rahlff

M. Wolf

J. Reinshagen

P. Gribbon

J.F. Imhoff

J. Silber

Antje Labes

B. Ellinger
Marine Fungi as Producers of Benzocoumarins, a New Class of Inhibitors of Glycogen-Synthase-Kinase 3β

Wiese, J. ., Imhoff, J. ., Gulder, T. ., Labes, A. ., & Schmaljohann, R. . (2017). Marine Fungi as Producers of Benzocoumarins, a New Class of Inhibitors of Glycogen-Synthase-Kinase 3β. Marine Drugs, 28, 14.
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2017

Journal Article

J. Wiese

J.F. Imhoff

T.A. Gulder

Antje Labes

R. Schmaljohann

2016

From Discovery to Production: Biotechnology of Marine Fungi for the Production of New Antibiotics

Silber, J. ., Kramer, A. ., Labes, A. ., & Tasdemir, D. . (2016). From Discovery to Production: Biotechnology of Marine Fungi for the Production of New Antibiotics. Marine Drugs, 14, 137. http://doi.org/10.3390/md14070137 (Original work published 2024)
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2016

Journal Article

Johanna Silber

Annemarie Kramer

Antje Labes

Deniz Tasdemir
Phylogenetic Relationship and Secondary Metabolite Production of Marine Fungi Producing the Cyclodepsipeptides Scopularide A and B

Kramer, A. ., Labes, A. ., & Imhoff, J. F. (2016). Phylogenetic Relationship and Secondary Metabolite Production of Marine Fungi Producing the Cyclodepsipeptides Scopularide A and B. Marine Biotechnology, 1–9. http://doi.org/10.1007/s10126-016-9707-7
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2016

Journal Article

Annemarie Kramer

Antje Labes

Johannes Imhoff

2015

Nature s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

Kunz, A. L., Labes, A. ., Wiese, J. ., Bruhn, T. ., Bringmann, G. ., & Imhoff, J. F. (2015). Nature s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain, Online-Ressource.
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2015

Journal Article

Anna Kunz

Antje Labes

Jutta Wiese

Torsten Bruhn

Gerhard Bringmann

Johannes Imhoff
Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant

Kramer, A. ., Beck, H. C., Kumar, A. ., Kristensen, L. P., Imhoff, J. F., & Labes, A. . (2015). Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant. PLoS ONE, 10, e0140047. Abgerufen von http://oceanrep.geomar.de/30275/

Abstract

The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. Here, we show the first proteome study of a marine fungus. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of the wild type strain and its mutant. For this purpose, an optimised protein extraction protocol was established. In total, 4759 proteins were identified. The central metabolic pathway of strain LF580 was mapped using the KEGG pathway analysis and GO annotation. Employing iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to limited nutrient availability in the wild type strain due to a strong pellet formation. This information can be applied for optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum
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2015

Journal Article

Annemarie Kramer

Hans Beck

Abhishek Kumar

Lars Kristensen

Johannes Imhoff

Antje Labes

Mutant strains

Proteomes

Protein extraction

Enzyme regulation

Fungi

Cell disruption

Protein metabolism

Biosynthesis
Antibiotic activity of microbial strains isolated from Mytilus edulis (Kiel Fjord, Baltic Sea)

Motiei, A. ., Wiese, J. ., Wenzel-Storjohann, A. ., Labes, A. ., Imhoff, J. F., & Gorokhova, E. . (2015). Antibiotic activity of microbial strains isolated from Mytilus edulis (Kiel Fjord, Baltic Sea). In Congress of European Microbiologists. Abgerufen von http://oceanrep.geomar.de/30441/
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2015

Conference Paper

A. Motiei

Jutta Wiese

Arlette Wenzel-Storjohann

Antje Labes

Johannes Imhoff

E. Gorokhova
Identification of Habitat-Specific Biomes of Aquatic Fungal Communities Using a Comprehensive Nearly Full-Length 18S rRNA Dataset Enriched with Contextual Data

Panzer, K. ., Yilmaz, P. ., Weiß, M. ., Reich, L. ., Richter, M. ., Wiese, J. ., … Reich, M. . (2015). Identification of Habitat-Specific Biomes of Aquatic Fungal Communities Using a Comprehensive Nearly Full-Length 18S rRNA Dataset Enriched with Contextual Data. PLoS ONE, 10, e0134377. Abgerufen von http://oceanrep.geomar.de/29516/

Abstract

Molecular diversity surveys have demonstrated that aquatic fungi are highly diverse, and that they play fundamental ecological roles in aquatic systems. Unfortunately, comparative studies of aquatic fungal communities are few and far between, due to the scarcity of adequate datasets. We combined all publicly available fungal 18S ribosomal RNA (rRNA) gene sequences with new sequence data from a marine fungi culture collection. We further enriched this dataset by adding validated contextual data. Specifically, we included data on the habitat type of the samples assigning fungal taxa to ten different habitat categories. This dataset has been created with the intention to serve as a valuable reference dataset for aquatic fungi including a phylogenetic reference tree. The combined data enabled us to infer fungal community patterns in aquatic systems. Pairwise habitat comparisons showed significant phylogenetic differences, indicating that habitat strongly affects fungal community structure. Fungal taxonomic composition differed considerably even on phylum and class level. Freshwater fungal assemblage was most different from all other habitat types and was dominated by basal fungal lineages. For most communities, phylogenetic signals indicated clustering of sequences suggesting that environmental factors were the main drivers of fungal community structure, rather than species competition. Thus, the diversification process of aquatic fungi must be highly clade specific in some cases.The combined data enabled us to infer fungal community patterns in aquatic systems. Pairwise habitat comparisons showed significant phylogenetic differences, indicating that habitat strongly affects fungal community structure. Fungal taxonomic composition differed considerably even on phylum and class level. Freshwater fungal assemblage was most different from all other habitat types and was dominated by basal fungal lineages. For most communities, phylogenetic signals indicated clustering of sequences suggesting that environmental factors were the main drivers of fungal community structure, rather than species competition. Thus, the diversification process of aquatic fungi must be highly clade specific in some cases.
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2015

Journal Article

Katrin Panzer

Pelin Yilmaz

Michael Weiß

Lothar Reich

Michael Richter

Jutta Wiese

Rolf Schmaljohann

Antje Labes

Johannes Imhoff

Frank Glöckner

Marlis Reich

Habitats

Fungal genetics

Phylogenetics

Fungi

Fungal structure

Fresh water

Phylogenetic analysis

Ribosomal RNA
Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae

Wu, B. ., Wiese, J. ., Labes, A. ., Kramer, A. ., Schmaljohann, R. ., & Imhoff, J. F. (2015). Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae. Marine Drugs, 13, 4617–4632. Abgerufen von http://oceanrep.geomar.de/29518/

Abstract

An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC50 value of 5.1 ($\pm$0.2) ?M and 3.2 ($\pm$0.4) \ensuremath\muM, respectively, against methicillin-resistant Staphylococcus aureus.
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2015

Journal Article

Bin Wu

Jutta Wiese

Antje Labes

Annemarie Kramer

Rolf Schmaljohann

Johannes Imhoff

MRSA

antibiotic

lindgomycin

marine fungi

marine natural products

2014

Malettinin E, an antibacterial and antifungal tropolone produced by a marine Cladosporium strain

Silber, J. ., Ohlendorf, B. ., Labes, A. ., Wenzel-Storjohann, A. ., Näther, C. ., & Imhoff, J. F. (2014). Malettinin E, an antibacterial and antifungal tropolone produced by a marine Cladosporium strain. Frontiers in Marine Science, 1, 1–6. Abgerufen von http://oceanrep.geomar.de/25470/ (Original work published 2024)

Abstract

The isolation and structure elucidation of malettinins A?C (1?3) along with the new malettinin E (4) are described. The compounds were produced by the fungus Cladosporium sp. strain KF501, which was isolated from the German Wadden Sea. The malettinins are built up of tropolone/dihydropyran ring structures linked to a furan ring. The structure elucidation of the isolated compounds was achieved by means of one- and two-dimensional NMR spectroscopy supported by mass and UV data. The relative configuration of 4 was determined on the basis of single-crystal X-ray diffraction analysis. 1?4 exhibited antibacterial and antifungal activities when profiled against Xanthomonas campestris and Trichophyton rubrum. The influence of the chemical structure of the furan ring and of configurational changes on biological activities was observed.
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2014

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Arlette Wenzel-Storjohann

Christian Näther

Johannes Imhoff

marinenaturalproducts

fungalnaturalproducts

antibacterial

antifungal

Cladosporium sp.

malettinin

tropolone

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