Silber, J. ., Ohlendorf, B. ., Erhard, A. ., Labes, A. ., & Imhoff, J. F. (2012). Marine fungi: a rich source for natural products. In SUBMARINER Blue Biotechnology Cooperation Event: New Strategies and Future Perspectives. Abgerufen von http://oceanrep.geomar.de/20272/
Staufenberger, T. ., Imhoff, J. F., & Labes, A. . (2012). First crenarchaeal chitinase found in Sulfolobus tokodaii. Microbiological Research, 167, 262–269. Abgerufen von http://oceanrep.geomar.de/12897/
Abstract
This is the first description of a functional chitinase gene within the crenarchaeotes. Here we report of the heterologues expression of the ORF BAB65950 from Sulfolobus tokodaii in E. coli. The resulting protein degraded chitin and was hence classified as chitinase (EC 3.2.4.14). The protein characterization revealed a specific activity of 75 mU/mg using colloidal chitin as substrate. The optimal activity of the enzyme was measured at pH 2.5 and 70 ?C, respectively. A dimeric enzyme configuration is proposed. According to amino acid sequence similarities chitinases are attributed to the two glycoside hydrolase families 18 and 19. The derived amino acid sequence of the S. tokodaii gene differed from sequences of these two glycoside hydrolase families. However, within a phylogenetic tree of protein sequences, the crenarchaeal sequence of S. tokodaii clustered in close proximity to members of the glycoside hydrolase family 18.
Mäntylä, M. . , V, Petersen, K. ., & Pfahl, D. . (2012). How many individuals to use in a QA task with fixed total effort?. In Proceedings of the 2012 ACM-IEEE International Symposium on Empirical Software Engineering and Measurement (S. 311–314).
Labes, A. . (2012). Meer Kosmetik. Neue Rohstoffe und Kosmetikkonzepte aus dem Meer ? was können marine mikrobielle Ressourcen dazu beitragen?. In L Oreal Pressekonferenz. Abgerufen von http://oceanrep.geomar.de/15352/
Goecke, F. R., Labes, A. ., Wiese, J. ., Schmaljohann, R. ., & Imhoff, J. F. (2012). Observation of bacteria over the surface of released oogonia from Fucus vesiculosus L (Phaeophyceae). Gayana Botanica, 69, 376–379. Abgerufen von http://oceanrep.geomar.de/19591/
Bin Ali, N. ., Petersen, K. ., & Mäntylä, M. . , V. (2012). Testing highly complex system of systems: an industrial case study. In Proceedings of the 2012 ACM-IEEE International Symposium on Empirical Software Engineering and Measurement (S. 211–220).
Podtschaske, B. ., & Friesdorf, W. . (2012). Sicherheit fördern durch interprofessionelle Kooperation. Mt -Medizintechnik. Organ Des Fachverbandes Biomedizinische Technik e.V. (fbmt), Des Bundesverbands Der Sachverständigen für Medizinprodukte BSM Sowie Der VDI-Gesellschaft Technologies of Life Sciences, Fachbereich Medizintechnik., 132(4), 143–145. Abgerufen von https://www.mt-medizintechnik.de/zeitschrift-mt-medizintechnik/
Faber, P. D.-I. T. ., & Klinke, L. . (2012). Einwirkungen auf das Bauwerk Offshore-Windenergieanlage. In Jahresausgabe Bauingenieur 2012. Springer VDI Verlag.
Goecke, F. R., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2012). Phylogenetic relationships among bacteria described from algae: Algae are a distinct source of new taxa. In 3. Latin-American Congress of Algal Biotechnology. Abgerufen von http://oceanrep.geomar.de/19547/