Paun, L. ., Kramer, A. ., Hihlal, E. ., Labes, A. ., Imhoff, J. F., & Kempken, F. . (2013). Higher yields of cyclodepsipetides from Scopulariopsis brevicaulis by random mutagenesis. In 27. Fungal Genetics Conference. Abgerufen von http://oceanrep.geomar.de/22016/
Abstract
The ascomycete Scopulariopsis brevicaulis, which was isolated from the marine sponge Tethya aurantium, produces two cyclodepsipeptides, scopularides A and B [1]. Both peptides exhibit activity against several tumor cell lines. Within the EU-project MARINE FUNGI (EU FP7, 265926) one of our aims is to enhance the production of these secondary metabolites. We are in the process to establish two ways of random mutagenesis by both UV radiation and transposon-mediated. To this end we created UV-mutants and a miniaturised screening method was developed. UV-radiation was performed at 312 nm and the survival rate was set to 1 \%. With this method a mutant library was established. To screen these mutants for higher secondary metabolites production, we developed a miniaturised screening method which includes decreased cultivation volume, fast extraction and an optimised LC-MS analysis format. Using the UV mutagenesis, we were able to identify several mutants with a higher scopularide production in comparison to the wild type. One of these mutants, which produces three times more biomass and more than double the amount of scopularide A, has been used for another round of mutation. Next generation sequencing is being employed to identify the molecular genetic basis of the observed mutations. In parallel we employ transposable elements to introduce mutants [2]. The impact of transposons on gene expression as well as their ability to cause major mutations within the genome or single genes makes them an interesting tool for random mutagenesis [3, 4, 5]. We employ the Vader transposon in its homologous host and found that Vader mostly integrates within or very close to genes. Thus it appears to be a useful tool for transposon-mediated mutagenesis in A. niger (6). At current we try to enhance its usability by modifying the Vader element.
Baca, D. ., Carlsson, B. ., Petersen, K. ., & Lundberg, L. . (2013). Improving software security with static automated code analysis in an industry setting. Software: Practice and Experience, 43, 259–279.
Labes, A. . (2013). Das Meer erforschen und Medizin finden. In Zukunft Meer - Ein Abend über das Erforschen, die Nutzung und den Schutz der Meere. Abgerufen von http://oceanrep.geomar.de/23010/
Labes, A. . (2013). Marine Biotechnologie in Schleswig-Holstein für die Apotheke aus dem Meer Technologietransfer für die Wirkstoffsuche aus marinen mikrobiellen Ressourcen. In Innovation Camp der FH Kiel. Abgerufen von http://oceanrep.geomar.de/23007/
Jabangwe, R. ., Petersen, K. ., & vSmite, D. \. (2013). Visualization of Defect Inflow and Resolution Cycles: Before, During and After Transfer. In 20th Asia-Pacific Software Engineering Conference.
Labes, A. ., Kaiser, H. ., & Tobin, S. . (2013). Das betörende Blühen der Titanenwurz. In Vorträge des Freundeskreis des Botanischen Gartens Kiel. Abgerufen von http://oceanrep.geomar.de/23008/
Nagel, K. ., Schneemann, I. ., Kajahn, I. ., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Beneficial effects of 2,4-diacetylphloroglucinol- producing pseudomonads on the marine alga Saccharina latissima. Aquatic Microbial Ecology, 67, 239–249. Abgerufen von http://oceanrep.geomar.de/19108/ (Original work published 2025)
Abstract
Pseudomonas strains were shown to be regularly associated with the brown macroalga Saccharina latissima from the Baltic Sea, studied over several years, and were identified as producers of the antimicrobially active compound 2,4-diacetylphloroglucinol. These findings support the assumption of a stable association between the Pseudomonas spp. strains and S. latissima in the Baltic Sea. The metabolite profile of the Pseudomonas spp. comprised monoacetylphloroglucinol, 2,4-diacetylphloroglucinol, pyoluteorin and several rhizoxins, which exhibited broad-spectrum antibiotic activities against Gram-positive and Gram-negative bacteria as well as against fungi. Because the antibiotic activities included the inhibition of the 2 algal pathogens Pseudoalteromonas elyakovii and Algicola bacteriolytica, we propose a beneficial effect of these marine pseudomonads on their host S. latissima.
Schlipf, D. ., Rettenmeier, A. ., Haizmann, F. ., Hofsäß, M. ., Courtney, M. ., & Cheng, P. W. (2012). Model based wind vector field reconstruction from lidar data. In German Wind Energy Conference (DEWEK). Bremen, Germany. http://doi.org/10.18419/opus-8136 (Original work published 2025)
Sandner, F. ., Schlipf, D. ., Matha, D. ., Seifried, R. ., & Cheng, P. W. (2012). Reduced nonlinear model of a spar-mounted floating wind turbine. In German Wind Energy Conference (DEWEK). Bremen, Germany. http://doi.org/10.18419/opus-4528 (Original work published 2025)