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2014

Natural products from marine fungi for the treatment of cancer

Imhoff, J. F., & Labes, A. . (2014). Natural products from marine fungi for the treatment of cancer. In 4. Gemeinsame Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) e. V. zusammen mit der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM) e. V. Abgerufen von http://oceanrep.geomar.de/26369/
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2014

Conference Paper

Johannes Imhoff

Antje Labes
A phenotypic screening approach to identify anti-cancer compounds derived from marine fungi

Ellinger, B. ., Landskron, J. ., Prashar, A. ., Silber, J. ., Wrigley, S. ., Tasken, K. ., … Gribbon, P. . (2014). A phenotypic screening approach to identify anti-cancer compounds derived from marine fungi. In ELRIG 2014 Research and Innovation - Impact into Cancer. Abgerufen von http://oceanrep.geomar.de/25550/
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2014

Conference Paper

B. Ellinger

J. Landskron

A. Prashar

Johanna Silber

S. Wrigley

K. Tasken

Antje Labes

P. Gribbon

2013

Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain

Silber, J. ., Ohlendorf, B. ., Labes, A. ., Erhard, A. ., & Imhoff, J. F. (2013). Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain. Marine Drugs, 11, 3309–3323. Abgerufen von http://oceanrep.geomar.de/21998/ (Original work published 2024)

Abstract

Bioactive compounds were detected in crude extracts of the fungus, Calcarisporium sp. KF525, which was isolated from German Wadden Sea water samples. Purification of the metabolites from the extracts yielded the five known polyesters, 15G256\ensuremath\alpha, \ensuremath\alpha-2, \ensuremath\beta, \ensuremath\beta-2 and \ensuremath\pi (1?5), and five new derivatives thereof, named calcarides A?E (6?10). The chemical structures of the isolated compounds were elucidated on the basis of one- and two-dimensional NMR spectroscopy supported by UV and HRESIMS data. The compounds exhibited inhibitory activities against Staphylococcus epidermidis, Xanthomonas campestris and Propionibacterium acnes. As the antibacterial activities were highly specific with regard to compound and test strain, a tight structure-activity relationship is assumed.
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2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Arlette Erhard

Johannes Imhoff

fungal natural products

Calcarisporium sp.

calcarides A?E

15G256 components

macrocyclic and linear polyesters

antibacterial activities

antibiotic

marine natural products
Calcaripeptides A?C, Cyclodepsipeptides from a Calcarisporium Strain

Silber, J. ., Ohlendorf, B. ., Labes, A. ., Näther, C. ., & Imhoff, J. F. (2013). Calcaripeptides A?C, Cyclodepsipeptides from a Calcarisporium Strain. Journal of Natural Products, 76, 1461–1467. Abgerufen von http://oceanrep.geomar.de/21997/ (Original work published Juli 2013)

Abstract

The isolation and structure elucidation of the novel calcaripeptides A (1), B (2), and C (3) and studies on their biosynthetic origin are described. The calcaripeptides were identified from Calcarisporium sp. strain KF525, which was isolated from the German Wadden Sea. Compounds 1?3 are macrocyclic structures composed of a proline and a phenylalanine residue as well as a nonpeptidic substructure. Structure elucidation was achieved by applying one- and two-dimensional NMR spectroscopy supported by high-resolution mass spectrometry. X-ray crystallography was performed to determine the relative configuration of 1. The absolute configuration of 1 was assigned by HPLC of the amino acids after hydrolysis of the molecule and derivatization with chiral agents. Studies on the biosynthesis by feeding 13C-labeled substrates revealed that the nonpeptidic part of 1 originates from acetate and l-methionine. The involvement of a hybrid between a polyketide synthase and a nonribosomal peptide synthetase in the biosynthesis of the calcaripeptides is discussed.
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2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Christian Näther

Johannes Imhoff
Higher yields of cyclodepsipetides from Scopulariopsis brevicaulis by random mutagenesis

Paun, L. ., Kramer, A. ., Hihlal, E. ., Labes, A. ., Imhoff, J. F., & Kempken, F. . (2013). Higher yields of cyclodepsipetides from Scopulariopsis brevicaulis by random mutagenesis. In 27. Fungal Genetics Conference. Abgerufen von http://oceanrep.geomar.de/22016/

Abstract

The ascomycete Scopulariopsis brevicaulis, which was isolated from the marine sponge Tethya aurantium, produces two cyclodepsipeptides, scopularides A and B [1]. Both peptides exhibit activity against several tumor cell lines. Within the EU-project MARINE FUNGI (EU FP7, 265926) one of our aims is to enhance the production of these secondary metabolites. We are in the process to establish two ways of random mutagenesis by both UV radiation and transposon-mediated. To this end we created UV-mutants and a miniaturised screening method was developed. UV-radiation was performed at 312 nm and the survival rate was set to 1 \%. With this method a mutant library was established. To screen these mutants for higher secondary metabolites production, we developed a miniaturised screening method which includes decreased cultivation volume, fast extraction and an optimised LC-MS analysis format. Using the UV mutagenesis, we were able to identify several mutants with a higher scopularide production in comparison to the wild type. One of these mutants, which produces three times more biomass and more than double the amount of scopularide A, has been used for another round of mutation. Next generation sequencing is being employed to identify the molecular genetic basis of the observed mutations. In parallel we employ transposable elements to introduce mutants [2]. The impact of transposons on gene expression as well as their ability to cause major mutations within the genome or single genes makes them an interesting tool for random mutagenesis [3, 4, 5]. We employ the Vader transposon in its homologous host and found that Vader mostly integrates within or very close to genes. Thus it appears to be a useful tool for transposon-mediated mutagenesis in A. niger (6). At current we try to enhance its usability by modifying the Vader element.
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2013

Conference Paper

Linda Paun

Annemarie Kramer

ElKbir Hihlal

Antje Labes

Johannes Imhoff

Frank Kempken
Das Meer erforschen und Medizin finden

Labes, A. . (2013). Das Meer erforschen und Medizin finden. In Zukunft Meer - Ein Abend über das Erforschen, die Nutzung und den Schutz der Meere. Abgerufen von http://oceanrep.geomar.de/23010/
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2013

Conference Paper

Antje Labes
Marine Biotechnologie in Schleswig-Holstein für die Apotheke aus dem Meer Technologietransfer für die Wirkstoffsuche aus marinen mikrobiellen Ressourcen

Labes, A. . (2013). Marine Biotechnologie in Schleswig-Holstein für die Apotheke aus dem Meer Technologietransfer für die Wirkstoffsuche aus marinen mikrobiellen Ressourcen. In Innovation Camp der FH Kiel. Abgerufen von http://oceanrep.geomar.de/23007/
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2013

Conference Paper

Antje Labes
Das betörende Blühen der Titanenwurz

Labes, A. ., Kaiser, H. ., & Tobin, S. . (2013). Das betörende Blühen der Titanenwurz. In Vorträge des Freundeskreis des Botanischen Gartens Kiel. Abgerufen von http://oceanrep.geomar.de/23008/
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2013

Conference Paper

Antje Labes

H. Kaiser

S. Tobin
Meeresschätze heben ? mit angewandter Forschung aus Norddeutschland

Labes, A. ., & Claussen, C. . (2013). Meeresschätze heben ? mit angewandter Forschung aus Norddeutschland. In Kieler Salon. Abgerufen von http://oceanrep.geomar.de/23005/
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2013

Conference Paper

Antje Labes

C. Claussen
Development of a miniaturised screening method for fungal mutants with enhanced production of specific natural compounds

Kramer, A. ., Paun, L. ., Labes, A. ., Kempken, F. ., & Imhoff, J. F. (2013). Development of a miniaturised screening method for fungal mutants with enhanced production of specific natural compounds. In VAAM Jahrestagung 2013. Abgerufen von http://oceanrep.geomar.de/22029/
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2013

Conference Paper

Annemarie Kramer

Linda Paun

Antje Labes

Frank Kempken

Johannes Imhoff

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2014

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