Publikationen

Autor*in

Jahr der Veröffentlichung

Publikationsformat

Keyword

Forschungsprojekt

Institut/Einrichtung

2014

Establishing the Secondary Metabolite Profile of a Marine Fungus: Tolypocladium geodes MF458

Kebede, B., Wrigley, S., Prashar, A., Rahlff, J., Ellinger, B., Silber, J., & Labes, A. (2014). Establishing the Secondary Metabolite Profile of a Marine Fungus: Tolypocladium geodes MF458. In ELRIG 2014 Research and Innovation - Impact into Cancer. Abgerufen von http://oceanrep.geomar.de/25552/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2014

Conference Paper

Bethlehem Kebede

Stephen Wrigley

Anjali Prashar

Janina Rahlff

Bernhard Ellinger

Johanna Silber

Antje Labes
Production of scopularide A in submerged culture with Scopulariopsis brevicaulis

Tamminen, A., Kramer, A., Labes, A., & Wiebe, M. G. (2014). Production of scopularide A in submerged culture with Scopulariopsis brevicaulis. Microbial cell factories, 13, 1–7. http://doi.org/10.1186/1475-2859-13-89
Suche auf Google Scholar

DOI Verweis
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2014

Journal Article

Anu Tamminen

Annemarie Kramer

Antje Labes

Marilyn Wiebe

2013

Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain

Silber, J., Ohlendorf, B., Labes, A., Erhard, A., & Imhoff, J. F. (2013). Calcarides A?E, Antibacterial Macrocyclic and Linear Polyesters from a Calcarisporium Strain. Marine Drugs, 11, 3309–3323. Abgerufen von http://oceanrep.geomar.de/21998/ (Original work published 2026)

Abstract

Bioactive compounds were detected in crude extracts of the fungus, Calcarisporium sp. KF525, which was isolated from German Wadden Sea water samples. Purification of the metabolites from the extracts yielded the five known polyesters, 15G256\ensuremath\alpha, \ensuremath\alpha-2, \ensuremath\beta, \ensuremath\beta-2 and \ensuremath\pi (1?5), and five new derivatives thereof, named calcarides A?E (6?10). The chemical structures of the isolated compounds were elucidated on the basis of one- and two-dimensional NMR spectroscopy supported by UV and HRESIMS data. The compounds exhibited inhibitory activities against Staphylococcus epidermidis, Xanthomonas campestris and Propionibacterium acnes. As the antibacterial activities were highly specific with regard to compound and test strain, a tight structure-activity relationship is assumed.
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Arlette Erhard

Johannes Imhoff

fungal natural products

Calcarisporium sp.

calcarides A?E

15G256 components

macrocyclic and linear polyesters

antibacterial activities

antibiotic

marine natural products
Calcaripeptides A-C, Cyclodepsipeptides from a Calcarisporium Strain

Silber, J., Ohlendorf, B., Labes, A., Näther, C., & Imhoff, J. F. (2013). Calcaripeptides A-C, Cyclodepsipeptides from a Calcarisporium Strain. Journal of Natural Products, 76, 1461–1467. Abgerufen von http://oceanrep.geomar.de/21997/ (Original work published Juli 2013)

Abstract

The isolation and structure elucidation of the novel calcaripeptides A (1), B (2), and C (3) and studies on their biosynthetic origin are described. The calcaripeptides were identified from Calcarisporium sp. strain KF525, which was isolated from the German Wadden Sea. Compounds 1?3 are macrocyclic structures composed of a proline and a phenylalanine residue as well as a nonpeptidic substructure. Structure elucidation was achieved by applying one- and two-dimensional NMR spectroscopy supported by high-resolution mass spectrometry. X-ray crystallography was performed to determine the relative configuration of 1. The absolute configuration of 1 was assigned by HPLC of the amino acids after hydrolysis of the molecule and derivatization with chiral agents. Studies on the biosynthesis by feeding 13C-labeled substrates revealed that the nonpeptidic part of 1 originates from acetate and l-methionine. The involvement of a hybrid between a polyketide synthase and a nonribosomal peptide synthetase in the biosynthesis of the calcaripeptides is discussed.
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Weiterführende Links
Profilbereich

Gesundheit und Life Sciences

Forschungsfeld

Nachhaltige Lebensmittelproduktion und Nutzung der Meere

Fachbereich

Fachbereich 2: Energy and Life Science
Filter Tags
2013

Journal Article

Johanna Silber

Birgit Ohlendorf

Antje Labes

Christian Näther

Johannes Imhoff
Bio-mining the microbial treasures of the ocean: Early drug discovery and models for entering pharmaceutical pipelines

Labes, A. (2013). Bio-mining the microbial treasures of the ocean: Early drug discovery and models for entering pharmaceutical pipelines. In SUBMARINER. Abgerufen von http://oceanrep.geomar.de/23002/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Antje Labes
Random mutagenesis in filamentous fungi for higher yields of secondary metabolites

Paun, L., Kramer, A., Labes, A., Imhoff, J. F., & Kempken, F. (2013). Random mutagenesis in filamentous fungi for higher yields of secondary metabolites. In 44. Annual Conference of the German Genetics Society (GfG). Abgerufen von http://oceanrep.geomar.de/22018/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Linda Paun

Annemarie Kramer

Antje Labes

Johannes Imhoff

Frank Kempken
Genome based methods for the exploration of natural products from marine fungi for the treatment of cancer

Labes, A. (2013). Genome based methods for the exploration of natural products from marine fungi for the treatment of cancer. In Tagung der Vereinigung für allgemeine und angewandte Mikrobiologie. Abgerufen von http://oceanrep.geomar.de/23000/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Antje Labes
Habitat specific provocation of secondary metabolite production by marine bacteria ? microbial communication and pharmaceutical use

Oesterwalbesloh, J., Wiese, J., Labes, A., & Imhoff, J. F. (2013). Habitat specific provocation of secondary metabolite production by marine bacteria ? microbial communication and pharmaceutical use. In VAAM Jahrestagung 2013. Abgerufen von http://oceanrep.geomar.de/20913/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Jan Oesterwalbesloh

Jutta Wiese

Antje Labes

Johannes Imhoff
Higher yields of cyclodepsipetides from Scopulariopsis brevicaulis by random mutagenesis

Paun, L., Kramer, A., Hihlal, E., Labes, A., Imhoff, J. F., & Kempken, F. (2013). Higher yields of cyclodepsipetides from Scopulariopsis brevicaulis by random mutagenesis. In 27. Fungal Genetics Conference. Abgerufen von http://oceanrep.geomar.de/22016/

Abstract

The ascomycete Scopulariopsis brevicaulis, which was isolated from the marine sponge Tethya aurantium, produces two cyclodepsipeptides, scopularides A and B [1]. Both peptides exhibit activity against several tumor cell lines. Within the EU-project MARINE FUNGI (EU FP7, 265926) one of our aims is to enhance the production of these secondary metabolites. We are in the process to establish two ways of random mutagenesis by both UV radiation and transposon-mediated. To this end we created UV-mutants and a miniaturised screening method was developed. UV-radiation was performed at 312 nm and the survival rate was set to 1 \%. With this method a mutant library was established. To screen these mutants for higher secondary metabolites production, we developed a miniaturised screening method which includes decreased cultivation volume, fast extraction and an optimised LC-MS analysis format. Using the UV mutagenesis, we were able to identify several mutants with a higher scopularide production in comparison to the wild type. One of these mutants, which produces three times more biomass and more than double the amount of scopularide A, has been used for another round of mutation. Next generation sequencing is being employed to identify the molecular genetic basis of the observed mutations. In parallel we employ transposable elements to introduce mutants [2]. The impact of transposons on gene expression as well as their ability to cause major mutations within the genome or single genes makes them an interesting tool for random mutagenesis [3, 4, 5]. We employ the Vader transposon in its homologous host and found that Vader mostly integrates within or very close to genes. Thus it appears to be a useful tool for transposon-mediated mutagenesis in A. niger (6). At current we try to enhance its usability by modifying the Vader element.
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Linda Paun

Annemarie Kramer

ElKbir Hihlal

Antje Labes

Johannes Imhoff

Frank Kempken
Das Meer erforschen und Medizin finden

Labes, A. (2013). Das Meer erforschen und Medizin finden. In Zukunft Meer - Ein Abend über das Erforschen, die Nutzung und den Schutz der Meere. Abgerufen von http://oceanrep.geomar.de/23010/
Suche auf Google Scholar
Exportformate

BibTeX

EndNote X3 XML

EndNote 7 XML

EndNote tagged

RIS
Filter Tags
2013

Conference Paper

Antje Labes

Publikationen

2014

Exportformate

Filter Tags

Exportformate

Filter Tags

2013

Abstract

Exportformate

Filter Tags

Abstract

Exportformate

Weiterführende Links

Filter Tags

Exportformate

Filter Tags

Exportformate

Filter Tags

Exportformate

Filter Tags

Exportformate

Filter Tags

Abstract

Exportformate

Filter Tags

Exportformate

Filter Tags