Labes, A. . (2012). Efficiency and material protection of a new cell disruption method for the extraction of bioactives from algae, bacteria and fungi. In Analytica. Abgerufen von http://oceanrep.geomar.de/15362/
Kurapati, N. ., Manyam, V. S. C., & Petersen, K. . (2012). Agile software development practice adoption survey. In International Conference on Agile Software Development (S. 16–30).
Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Bacteria associations with co-occurring macroalgae: Host, epiphyte and environmental factors. European Journal of Phycology, 46, 45–50. Abgerufen von http://oceanrep.geomar.de/19586/
Abstract
Bacteria associated with 2 macroalgae growing in the Kiel Fjord (Baltic Sea) were investigated seasonally over two years by scanning electron microscopy and cultivation methods. Seasonal variations and significant differences between both macroalgal species with respect to the associated bacteria were observed. 166 bacterial strains were isolated from the macroalgae and classified by phylogenetic analysis of 16S rRNA gene sequences. The strains formed 82 phylotypes according to sequence similarities of499.0\%. Samples from Fucus vesiculosus revealed 43 and from Delesseria sanguinea 57 bacterial phylotypes. They affiliated to Actinobacteria, Alphaproteobacteria, Bacilli, Betaproteobacteria, Flavobacteria, Gammaproteobacteria, and Sphingobacteria. Following the assumption that chemical interactions rule the bacteria-macroalga associations, we tested the biological activity of both macroalgae and isolated bacterial strains against a panel comprising standard and ecologically relevant microorganisms (Gram-positive and Gram-negative bacteria, including macroalgal pathogens and surface associated strains, and a yeast). Both macroalgae presented inhibitory activity against at least one microorganism, but overall the inhibitory activities were low. In contrast, all extracts stimulated growth of many of the tested bacteria. While growth stimulation was common concerning bacteria associated with macroalgae, no stimulation was shown for the standard set of bacteria (with exception of one case of stimulation of the plant pathogen Erwinia amylovora). Regarding the potential biological activity of microorganisms associated with macroalgae, more than 60\% of the bacterial phylotypes inhibited the growth of at least one microorganism. A higher proportion of strains showed antimicrobial activity against the set of bacteria associated with macroalgae as compared to standard set of microorganisms. Certain phylotypes are consistently found as epiphytes, suggesting their specific association with macroalgae. The presented positive and negative effects of macroalgal extracts on growth of macroalga-associated bacteria, significant bacterial activity against macroalgal pathogens and competitors, and potential degradative capabilities support the assumption that complex chemical interactions shape the bacteria-macroalga relationships.
Labes, A. ., Oesterwalbesloh, J. ., & Imhoff, J. F. (2012). Secondary metabolites from deep sea bacteria: an efficient route for biodiscovery from extreme habitats. In Extremophiles. Abgerufen von http://oceanrep.geomar.de/15367/
Tuschy, I. ., Render, M. ., & Vogelsang, A. . (2012). Planung, Einsatz und Entwicklung thermischer Kraftwerke mittlerer Größe unter neuen energiewirtschaftlichen Rahmenbedingungen. In M. . Beckmann & A. . Hurtado (Hrsg.), Kraftwerkstechnik (Bd. 4). Neuruppin: TK-Verlag.
Staufenberger, T. ., Imhoff, J. F., & Labes, A. . (2012). First crenarchaeal chitinase found in Sulfolobus tokodaii. Microbiological Research, 167, 262–269. Abgerufen von http://oceanrep.geomar.de/12897/
Abstract
This is the first description of a functional chitinase gene within the crenarchaeotes. Here we report of the heterologues expression of the ORF BAB65950 from Sulfolobus tokodaii in E. coli. The resulting protein degraded chitin and was hence classified as chitinase (EC 3.2.4.14). The protein characterization revealed a specific activity of 75 mU/mg using colloidal chitin as substrate. The optimal activity of the enzyme was measured at pH 2.5 and 70 ?C, respectively. A dimeric enzyme configuration is proposed. According to amino acid sequence similarities chitinases are attributed to the two glycoside hydrolase families 18 and 19. The derived amino acid sequence of the S. tokodaii gene differed from sequences of these two glycoside hydrolase families. However, within a phylogenetic tree of protein sequences, the crenarchaeal sequence of S. tokodaii clustered in close proximity to members of the glycoside hydrolase family 18.
Huth, G. ., & Löhlein, B. . (2012). Zahnelement zum Einsatz in einem Ständer für einen Elektromotor sowie entsprechendes Verfahren zur Herstellung eines solchen Zahnelements. Abgerufen von https://depatisnet.dpma.de/DepatisNet/depatisnet?action=bibdat&docid=DE102010040245A1
Löhlein, B. . (2012). Entwurf und Optimierung von hochtourigen permanentmagneterregten Synchronmotoren in SMC-Technologie. Aachen: Shaker Verlag. Abgerufen von https://www.shaker.eu/en/content/catalogue/index.asp?ID=8&ISBN=978-3-8440-1412-9