Petersen, K. . (2012). A palette of lean indicators to detect waste in software maintenance: A case study. In International Conference on Agile Software Development (S. 108–122).
Goecke, F. R., Wiese, J. ., Nunez, A. ., Labes, A. ., Imhoff, J. F., & Neuhauser, S. . (2012). A Novel Phytomyxean Parasite Associated with Galls on the Bull-Kelp Durvillaea antarctica (Chamisso) Hariot. PLoS ONE, 7, e45358. Abgerufen von http://oceanrep.geomar.de/19148/
Abstract
Durvillaea antarctica (Fucales, Phaeophyceae) is a large kelp of high ecological and economic significance in the Southern Hemisphere. In natural beds along the central coast of Chile (Pacific Ocean), abnormal growth characterized by evident gall development and discolorations of the fronds/thallus was observed. Analysing these galls by light microscopy and scanning electron microscopy revealed the presence of endophytic eukaryotes showing typical characteristics for phytomyxean parasites. The parasite developed within enlarged cells of the subcortical tissue of the host. Multinucleate plasmodia developed into many, single resting spores. The affiliation of this parasite to the Phytomyxea (Rhizaria) was supported by 18S rDNA data, placing it within the Phagomyxida. Similar microorganisms were already reported once 23 years ago, indicating that these parasites are persistent and widespread in D. antarctica beds for long times. The symptoms caused by this parasite are discussed along with the ecological and economic consequences. Phytomyxean parasites may play an important role in the marine ecosystem, but they remain understudied in this environment. Our results demonstrate for the first time the presence of resting spores in Phagomyxida, an order in which resting spores were thought to be absent making this the first record of a phagomyxean parasite with a complete life cycle so far, challenging the existing taxonomic concepts within the Phytomyxea. The importance of the here described resting spores for the survival and ecology of the phagomyxid parasite will be discussed together with the impact this parasite may have on the strongest seaweed of the world , which is an important habitat forming and economic resource from the Southern Hemisphere.
Goecke, F. R., Thiel, V. ., Wiese, J. ., Labes, A. ., & Imhoff, J. F. (2012). Phylogenetic relationships among bacteria described from algae: Distinct source of new taxa. In Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie. Abgerufen von http://oceanrep.geomar.de/19548/
Labes, A. . (2012). Efficiency and material protection of a new cell disruption method for the extraction of bioactives from algae, bacteria and fungi. In Analytica. Abgerufen von http://oceanrep.geomar.de/15362/
Kurapati, N. ., Manyam, V. S. C., & Petersen, K. . (2012). Agile software development practice adoption survey. In International Conference on Agile Software Development (S. 16–30).
Goecke, F. R., Labes, A. ., Wiese, J. ., & Imhoff, J. F. (2012). Bacteria associations with co-occurring macroalgae: Host, epiphyte and environmental factors. European Journal of Phycology, 46, 45–50. Abgerufen von http://oceanrep.geomar.de/19586/
Abstract
Bacteria associated with 2 macroalgae growing in the Kiel Fjord (Baltic Sea) were investigated seasonally over two years by scanning electron microscopy and cultivation methods. Seasonal variations and significant differences between both macroalgal species with respect to the associated bacteria were observed. 166 bacterial strains were isolated from the macroalgae and classified by phylogenetic analysis of 16S rRNA gene sequences. The strains formed 82 phylotypes according to sequence similarities of499.0\%. Samples from Fucus vesiculosus revealed 43 and from Delesseria sanguinea 57 bacterial phylotypes. They affiliated to Actinobacteria, Alphaproteobacteria, Bacilli, Betaproteobacteria, Flavobacteria, Gammaproteobacteria, and Sphingobacteria. Following the assumption that chemical interactions rule the bacteria-macroalga associations, we tested the biological activity of both macroalgae and isolated bacterial strains against a panel comprising standard and ecologically relevant microorganisms (Gram-positive and Gram-negative bacteria, including macroalgal pathogens and surface associated strains, and a yeast). Both macroalgae presented inhibitory activity against at least one microorganism, but overall the inhibitory activities were low. In contrast, all extracts stimulated growth of many of the tested bacteria. While growth stimulation was common concerning bacteria associated with macroalgae, no stimulation was shown for the standard set of bacteria (with exception of one case of stimulation of the plant pathogen Erwinia amylovora). Regarding the potential biological activity of microorganisms associated with macroalgae, more than 60\% of the bacterial phylotypes inhibited the growth of at least one microorganism. A higher proportion of strains showed antimicrobial activity against the set of bacteria associated with macroalgae as compared to standard set of microorganisms. Certain phylotypes are consistently found as epiphytes, suggesting their specific association with macroalgae. The presented positive and negative effects of macroalgal extracts on growth of macroalga-associated bacteria, significant bacterial activity against macroalgal pathogens and competitors, and potential degradative capabilities support the assumption that complex chemical interactions shape the bacteria-macroalga relationships.
Labes, A. ., Oesterwalbesloh, J. ., & Imhoff, J. F. (2012). Secondary metabolites from deep sea bacteria: an efficient route for biodiscovery from extreme habitats. In Extremophiles. Abgerufen von http://oceanrep.geomar.de/15367/
Tuschy, I. ., Render, M. ., & Vogelsang, A. . (2012). Planung, Einsatz und Entwicklung thermischer Kraftwerke mittlerer Größe unter neuen energiewirtschaftlichen Rahmenbedingungen. In M. . Beckmann & A. . Hurtado (Hrsg.), Kraftwerkstechnik (Bd. 4). Neuruppin: TK-Verlag.