Publikationen

2014

• Windenergieanlagen mit verbesserter Leistungsreservenbereithaltung DE102013203540

  Geisler, J. ., Schrader, S. ., Bluhm, R. ., & Petsche, M. . (2014). Windenergieanlagen mit verbesserter Leistungsreservenbereithaltung DE102013203540. Germany: Senvion GmbH. (Original work published 2014)

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    • Geisler, Jens

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  • 2014
  • Patent
  • • Jens Geisler
    • Stefan Schrader
    • R. Bluhm
    • M. Petsche

• Development and Validation of a Fast and Optimized Screening Method for Enhanced Production of Secondary Metabolites Using the Marine Scopulariopsis brevicaulis Strain LF580 Producing Anti-Cancer Active Scopularide A and B

  Kramer, A. ., Paun, L. ., Imhoff, J. F., Kempken, F. ., & Labes, A. . (2014). Development and Validation of a Fast and Optimized Screening Method for Enhanced Production of Secondary Metabolites Using the Marine Scopulariopsis brevicaulis Strain LF580 Producing Anti-Cancer Active Scopularide A and B. PLoS ONE, 9, e103320. Abgerufen von http://oceanrep.geomar.de/25471/ (Original work published Juli 2014)

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  Natural compounds from marine fungi are an excellent source for the discovery and development of new drug leads. The distinct activity profiles of the two cyclodepsipeptides scopularide A and B against cancer cell lines set their marine producer strain Scopulariopsis brevicaulis LF580 into the focus of the EU project MARINE FUNGI. One of the main goals was the development of a sustainable biotechnological production process for these compounds. The secondary metabolite production of strain LF580 was optimized by random mutagenesis employing UV radiation. For a fast and reliable detection of the intracellular secondary metabolite production level, a miniaturized bioactivity-independent screening method was developed, as the random mutagenesis yielded a large number of mutants to be analysed quantitatively and none of the existing hyphenated bioassay-dependent screening systems could be applied. The method includes decreased cultivation volume, a fast extraction procedure as well as an optimized LC-MS analysis. We show that deviation could be specifically reduced at each step of the process: The measuring deviation during the analysis could be minimized to 5\% and technical deviation occurring in the downstream part to 10?15\%. Biological variation during the cultivation process still has the major influence on the overall variation. However, the approach led to a 10-fold reduction of time and similar effects on costs and effort compared to standard reference screening methods. The method was applied to screen the UV-mutants library of Scopulariopsis brevicaulis LF580. For validation purposes, the occurring variations in the miniaturized scale were compared to those in the classical Erlenmeyer flask scale. This proof of concept was performed using the wild type strain and 23 randomly selected mutant strains. One specific mutant strain with an enhanced production behavior could be obtained.

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  • 2014
  • Journal Article
  • • Annemarie Kramer
    • Linda Paun
    • Johannes Imhoff
    • Frank Kempken
    • Antje Labes
  • • Drug metabolism
    • Fungi
    • Library screening
    • Linear regression analysis
    • Liquid chromatography-mass spectrometry
    • Mutant strains Secondary metabolism
    • Ultraviolet radiation

• Exploiting the didactic possibilities of low-fi simulation in virtual bridge-team communication exercises

  Noble, A. ., Björkroth, P. ., & John, P. . (2014). Exploiting the didactic possibilities of low-fi simulation in virtual bridge-team communication exercises. In International Maritime English Conference (IMLA-IMEC) (S. 16). Terschelling (The Netherlands): Maritime Institute Willem Barentsz . Abgerufen von https://www.pfri.uniri.hr/bopri/IMEC_Proceedings/PDF/IMEC26.pdf (Original work published Juli 2014)

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  The research paper is the second in a series of studies on an improved use of the Standard Marine Communication Phrases (SMCP) during an international low-fi simulation exercise in a classroom setting. Following a briefing on the use of relevant SMCP by means of an e-learning platform, students of Maritime English at several maritime universities participated in a virtual bridge team exercise. The communication patterns employed in their situational assessment and decision making were compared to previous low-fi simulations whereby participants had not received any briefing on the use of SMCP prior to the exercise [1] (cf. John et al., 2013). Based on the different features observed in both exercise settings, the scope for using a constructivist learning environment as offered by low-fi simulations is discussed and possibilities for an integral pedagogical approach towards teaching standard phraseology are outlined.

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    • John, Peter

  • Fachbereich

    • Fachbereich 3: Information und Kommunikation

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  • 2014
  • Conference Paper
  • • Alison Noble
    • Peter Björkroth
    • Peter John
  • • Bridge team communication
    • Low-fi simulation
    • Standard Marine Communication Phrases
    • Integral pedagogical approach

• Windenergieanlage und Verfahren zum Regeln des elektrischen Moments einer Windenergieanlage bei einem Netzfehler DE102012224067

  Geisler, J. ., Schrader, S. ., & Fortmann, J. . (2014). Windenergieanlage und Verfahren zum Regeln des elektrischen Moments einer Windenergieanlage bei einem Netzfehler DE102012224067. Germany: Senvion GmbH. (Original work published Juni 2014)

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    • Geisler, Jens

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  • 2014
  • Patent
  • • Jens Geisler
    • Stefan Schrader
    • J. Fortmann

• Nature?s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain

  Kunz, A. L., Labes, A. ., Wiese, J. ., Bruhn, T. ., Bringmann, G. ., & Imhoff, J. F. (2014). Nature?s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain. Marine Drugs, 12, 1699–1714. Abgerufen von http://oceanrep.geomar.de/24372/ (Original work published März 2014)

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  Eight streptophenazines (A?H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I?K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I?K) also showed moderate activities against PDE 4B.

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  • 2014
  • Journal Article
  • • Anna Kunz
    • Antje Labes
    • Jutta Wiese
    • Torsten Bruhn
    • Gerhard Bringmann
    • Johannes Imhoff
  • • marine natural product
    • phenazine
    • Baltic Sea
    • Streptomyces
    • Halichondria panicea
    • HPLC-DAD/MS
    • fermentation
    • PDE 4
    • antibiotic

• Towards a hybrid testing process unifying exploratory testing and scripted testing

  Shah, S. M. A., Gencel, C. ., Alvi, U. S., & Petersen, K. . (2014). Towards a hybrid testing process unifying exploratory testing and scripted testing. Journal of Software: Evolution and Process, 26, 220–250.

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    • Petersen, Kai

  • Abteilung/Einrichtung

    • FLAIR - Flensburg Artificial Intelligence Research

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  • 2014
  • Journal Article
  • • Syed Shah
    • Cigdem Gencel
    • Usman Alvi
    • Kai Petersen

• Comparing a hybrid testing process with scripted and exploratory testing: an experimental study with practitioners

  Shah, S. M. A., Alvi, U. S., Gencel, C. ., & Petersen, K. . (2014). Comparing a hybrid testing process with scripted and exploratory testing: an experimental study with practitioners. In International Conference on Agile Software Development (S. 187–202).

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    • Petersen, Kai

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    • FLAIR - Flensburg Artificial Intelligence Research

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  • 2014
  • Conference Paper
  • • Syed Shah
    • Usman Alvi
    • Cigdem Gencel
    • Kai Petersen

• Presentations of the scientific briefing "New frontiers in Blue Biotechnology - Research and SME Alliances in Early Drug Discovery"

  Labes, A. ., & Imhoff, J. F. (2014). Presentations of the scientific briefing "New frontiers in Blue Biotechnology - Research and SME Alliances in Early Drug Discovery". In European Parliament. Abgerufen von http://oceanrep.geomar.de/26366/

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  • 2014
  • Conference Paper
  • • Antje Labes
    • Johannes Imhoff

• Stochastic Bandwidth Estimation in Networks With Random Service

  Lübben, R. ., Fidler, M. ., & Liebeherr, J. . (2014). Stochastic Bandwidth Estimation in Networks With Random Service. IEEE/ACM/Transactions/on/Networking, 22, 484–497. http://doi.org/10.1109/TNET.2013.2261914

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    • Lübben, Ralf

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    • Fachbereich 4: Wirtschaft

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  • 2014
  • Journal Article
  • • Ralf Lübben
    • Markus Fidler
    • Jörg Liebeherr

• An experimental evaluation of test driven development vs. test-last development with industry professionals

  Munir, H. ., Wnuk, K. ., Petersen, K. ., & Moayyed, M. . (2014). An experimental evaluation of test driven development vs. test-last development with industry professionals. In Proceedings of the 18th International Conference on Evaluation and Assessment in Software Engineering (S. 1–10).

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    • Petersen, Kai

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    • FLAIR - Flensburg Artificial Intelligence Research

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  • 2014
  • Conference Paper
  • • Hussan Munir
    • Krzysztof Wnuk
    • Kai Petersen
    • Misagh Moayyed