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2013

Development of a miniaturised screening method for fungal mutants with enhanced production of specific natural compounds

Kramer, A., Paun, L., Labes, A., Kempken, F., & Imhoff, J. F. (2013). Development of a miniaturised screening method for fungal mutants with enhanced production of specific natural compounds. In VAAM Jahrestagung 2013. Abgerufen von http://oceanrep.geomar.de/22029/
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2013

Conference Paper

Annemarie Kramer

Linda Paun

Antje Labes

Frank Kempken

Johannes Imhoff
Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae

Goecke, F. R., Thiel, V., Wiese, J., Labes, A., & Imhoff, J. F. (2013). Algae as important environment for bacteria - phylogenetic relationships among species isolated from algae. Phycologia, 52, 14–24. Abgerufen von http://oceanrep.geomar.de/19593/

Abstract

Bacteria are an inherent part of the biotic environment of algae. Recent investigations revealed that bacterial communities associated with algae were generally highly host specific. Several new bacterial species and genera were isolated from algae, which suggested that algae were an interesting environment for discovery of new bacterial taxa; however, the distribution of the different phylogenetic groups among those isolates remained unclear, and this information could help to explain specific associations. We conducted a phylogenetic study based on 16S rRNA gene sequences available in GenBank, including 101 validly described bacterial species that were isolated from eukaryotic macro- and micro-algae from marine and freshwater environments. These species were distributed among six bacterial phyla, including: Bacteroidetes (42 species), Proteobacteria (36 species), and Firmicutes, Actinobacteria, Verrucomicrobia and Planctomycetes (23 species). Bacterial species and strains that carried out similar metabolic functions were found to colonize similar algal taxa or algal groups. This assumption was supported by information available from bacterial species descriptions: (1) Most of the bacteria described from microalgae grouped into the Roseobacter clade (Alphaproteobacteria), which indicated that members of this group were well adapted for life in close association with phytoplankton; and (2) 32\% of the bacterial species, mainly isolates from macroalgae, were able to decompose macroalgal polysaccharides. Because algal-bacterial association are still under-studied in various algal groups, we expect a great number of new bacterial taxa to be discovered in the future.
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2013

Journal Article

Franz Goecke

Vera Thiel

Jutta Wiese

Antje Labes

Johannes Imhoff

Enzyme

Macroalgae

Microbe-alga

Phytoplankton

DINOFLAGELLATE GYMNODINIUM-CATENATUM

MARINE ROSEOBACTER LINEAGE

DISSOLVED ORGANIC-MATTER

IN-SITU HYBRIDIZATION

EPIPHYTIC BACTERIAL

LAMINARIA-JAPONICA

GEN. NOV.

COMMUNITY COMPOSITION

ALEXANDRIUM SPP.

BROWN ALGA
Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea

Goecke, F. R., Labes, A., Wiese, J., & Imhoff, J. F. (2013). Phylogenetic analysis and antibiotic activity of bacteria isolated from the surface of two co-occurring macroalgae from the Baltic Sea. European Journal of Phycology, 48, 47–60. Abgerufen von http://oceanrep.geomar.de/19592/

Abstract

Bacteria associated with Fucus vesiculosus and Delesseria sanguinea, two macroalgae from the Kiel Fjord were investigated seasonally over two years by cultivation-based methods. A total of 166 bacterial strains were isolated from the macroalgae, affiliated to seven classes of bacteria (Actinobacteria, Bacilli, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Cytophagia and Flavobacteria). According to 16S rRNA gene sequence similarities they were arranged in 82 phylotypes of\ensuremath>99.0\% sequence identity. Assuming that chemical factors rule the bacteriamacroalga and bacteriabacteria interactions on algal surfaces, we tested the antibiotic activity of the bacterial isolates not only against a panel of four standard test organisms (Bacillus subtilis, Candida glabrata, Escherichia coli and Staphylococcus lentus) but also four macroalga-associated microorganisms: Algicola bacteriolytica and Pseudoalteromonas elyakovii (macroalgal pathogens), and Bacillus algicola and Formosa algae (strains associated with algal surfaces). Organic extracts of more than 51\% of the isolates from the two macroalgae inhibited the growth of at least one of the tested microorganisms. As much as 46\% and 45\% of the isolates derived from F. vesiculosus and D. sanguinea, respectively, showed antimicrobial activity against the set of macroalga-associated bacteria, compared with 13 and 19\% against a standard set of microorganisms. High antibacterial activity against macroalgal pathogens and bacterial competitors support the assumption that complex chemical interactions shape the relationships of bacteria associated with macroalgae and suggest that these bacteria are a rich source of antimicrobial metabolites.
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2013

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff

antimicrobial activities

bacterial cultures

chemical associations

Delesseria

epiphytic bacteria

Fucus

Kiel

macroalgabacteria associations
Early drug discovery: Models for Entering Pharmaceutical Pipelines

Labes, A. (2013). Early drug discovery: Models for Entering Pharmaceutical Pipelines. In 2. Deutsch-Russisches Forum Biotechnologie. Abgerufen von http://oceanrep.geomar.de/23001/
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2013

Conference Paper

Antje Labes
Bio-mining the microbial treasures of the ocean: Early drug discovery and models for entering pharmaceutical pipelines

Labes, A. (2013). Bio-mining the microbial treasures of the ocean: Early drug discovery and models for entering pharmaceutical pipelines. In SUBMARINER. Abgerufen von http://oceanrep.geomar.de/23002/
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2013

Conference Paper

Antje Labes
Random mutagenesis in filamentous fungi for higher yields of secondary metabolites

Paun, L., Kramer, A., Labes, A., Imhoff, J. F., & Kempken, F. (2013). Random mutagenesis in filamentous fungi for higher yields of secondary metabolites. In 44. Annual Conference of the German Genetics Society (GfG). Abgerufen von http://oceanrep.geomar.de/22018/
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2013

Conference Paper

Linda Paun

Annemarie Kramer

Antje Labes

Johannes Imhoff

Frank Kempken
Genome based methods for the exploration of natural products from marine fungi for the treatment of cancer

Labes, A. (2013). Genome based methods for the exploration of natural products from marine fungi for the treatment of cancer. In Tagung der Vereinigung für allgemeine und angewandte Mikrobiologie. Abgerufen von http://oceanrep.geomar.de/23000/
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2013

Conference Paper

Antje Labes

2012

Beneficial effects of 2,4-diacetylphloroglucinol- producing pseudomonads on the marine alga Saccharina latissima

Nagel, K., Schneemann, I., Kajahn, I., Labes, A., Wiese, J., & Imhoff, J. F. (2012). Beneficial effects of 2,4-diacetylphloroglucinol- producing pseudomonads on the marine alga Saccharina latissima. Aquatic Microbial Ecology, 67, 239–249. Abgerufen von http://oceanrep.geomar.de/19108/ (Original work published 2026)

Abstract

Pseudomonas strains were shown to be regularly associated with the brown macroalga Saccharina latissima from the Baltic Sea, studied over several years, and were identified as producers of the antimicrobially active compound 2,4-diacetylphloroglucinol. These findings support the assumption of a stable association between the Pseudomonas spp. strains and S. latissima in the Baltic Sea. The metabolite profile of the Pseudomonas spp. comprised monoacetylphloroglucinol, 2,4-diacetylphloroglucinol, pyoluteorin and several rhizoxins, which exhibited broad-spectrum antibiotic activities against Gram-positive and Gram-negative bacteria as well as against fungi. Because the antibiotic activities included the inhibition of the 2 algal pathogens Pseudoalteromonas elyakovii and Algicola bacteriolytica, we propose a beneficial effect of these marine pseudomonads on their host S. latissima.
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2012

Journal Article

Kerstin Nagel

Imke Schneemann

Inga Kajahn

Antje Labes

Jutta Wiese

Johannes Imhoff

Pseudomonas protegens ? Alga?bacteria association ? 2

4-diacetylphloroglucinol ? Pyoluteorin ? Rhizoxin ? Brown macroalgae

RESISTANT STAPHYLOCOCCUS-AUREUS

FLUORESCENT PSEUDOMONAS

LAMINARIA-SACCHARINA

SECONDARY METABOLITES

IN-VITRO

BACTERIA

PF-5

JAPONICA

STRAINS

AGENT
Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea

Goecke, F. R., Labes, A., Wiese, J., & Imhoff, J. F. (2012). Dual effect of macroalgal extracts on growth of bacteria in Western Baltic Sea. Revista de Biologia Marina y Oceanografia, 47, 75–86. Abgerufen von http://oceanrep.geomar.de/14347/

Abstract

It is assumed that the biological characteristics of the bacterial strains used in bioactivity tests have strong influences on their susceptibility against antibacterial compounds. Therefore, the selection of bacterial test strains may rush conclusions on the effect of macroalgal extracts and metabolites on bacteria. To proof this assumption, we have analysed the biological activities of crude extracts of 16 macroalgae from the coastal waters of Kiel Fjord (Germany), and tested their effect against a panel of 10 microorganisms comprising 5 standard test strains of bacteria and 5 macroalga-associated bacteria. Fourteen macroalgae (88\%) displayed antibacterial activity against at least one of the test strains. Despite the high proportion of extracts exhibiting antimicrobial activity, only 3 strains of the standard set were susceptible to macroalgal extracts and the overall activities were low (less than 80\% of inhibition). Most of active extracts inhibited Bacillus subtilis, while no inhibition effects were found against Erwinia amylovora, Escherichia coli, and the macroalga-associated bacteria. In contrast, all extracts produced stimulatory growth effects of at least two of the tested bacteria. While growth stimulation of standard set of bacteria was rare (22.5\% of total tests) with exception of plant pathogen Erwinia amylovora and two cases of Staphylococcus lentus, it was common among bacteria associated with macroalgae (77.5\%), especially Bacillus algicola, Pseudomonas marincola and both algal-pathogenic bacteria. This study demonstrates that macroalgal extracts can display different effects, i.e., inhibition or stimulation of bacterial growth depending on the origin of the test strains, which are derived from a standard panel or from the marine environment, respectively.
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2012

Journal Article

Franz Goecke

Antje Labes

Jutta Wiese

Johannes Imhoff

Microbiology

Marine algae

antibacterial activities

stimulation of bacterial growth

Kiel fjord
A Novel Phytomyxean Parasite Associated with Galls on the Bull-Kelp Durvillaea antarctica (Chamisso) Hariot

Goecke, F. R., Wiese, J., Nunez, A., Labes, A., Imhoff, J. F., & Neuhauser, S. (2012). A Novel Phytomyxean Parasite Associated with Galls on the Bull-Kelp Durvillaea antarctica (Chamisso) Hariot. PLoS ONE, 7, e45358. Abgerufen von http://oceanrep.geomar.de/19148/

Abstract

Durvillaea antarctica (Fucales, Phaeophyceae) is a large kelp of high ecological and economic significance in the Southern Hemisphere. In natural beds along the central coast of Chile (Pacific Ocean), abnormal growth characterized by evident gall development and discolorations of the fronds/thallus was observed. Analysing these galls by light microscopy and scanning electron microscopy revealed the presence of endophytic eukaryotes showing typical characteristics for phytomyxean parasites. The parasite developed within enlarged cells of the subcortical tissue of the host. Multinucleate plasmodia developed into many, single resting spores. The affiliation of this parasite to the Phytomyxea (Rhizaria) was supported by 18S rDNA data, placing it within the Phagomyxida. Similar microorganisms were already reported once 23 years ago, indicating that these parasites are persistent and widespread in D. antarctica beds for long times. The symptoms caused by this parasite are discussed along with the ecological and economic consequences. Phytomyxean parasites may play an important role in the marine ecosystem, but they remain understudied in this environment. Our results demonstrate for the first time the presence of resting spores in Phagomyxida, an order in which resting spores were thought to be absent making this the first record of a phagomyxean parasite with a complete life cycle so far, challenging the existing taxonomic concepts within the Phytomyxea. The importance of the here described resting spores for the survival and ecology of the phagomyxid parasite will be discussed together with the impact this parasite may have on the strongest seaweed of the world , which is an important habitat forming and economic resource from the Southern Hemisphere.
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2012

Journal Article

Franz Goecke

Jutta Wiese

Alejandra Nunez

Antje Labes

Johannes Imhoff

Sigrid Neuhauser

SP-NOV

LESSONIA-NIGRESCENS

MARINE MACROALGAE

CENTRAL CHILE

NEW-ZEALAND

PHAEOPHYTA

RHODOPHYTA

ALGAE

DURVILLEALES

INFECTIONS

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2013

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