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2008

Novel members of glycoside hydrolase family 13 derived from environmental DNA

Labes, A. ., Karlsson, N. ., Fridjonsson, O. ., Turner, P. ., Hreggvidson, G. ., Kristjansson, J. ., … Schönheit, P. . (2008). Novel members of glycoside hydrolase family 13 derived from environmental DNA. Applied and Environmental Microbiology, 74, 1914–1921. Abgerufen von http://oceanrep.geomar.de/7384/
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2008

Journal Article

Antje Labes

Nordberg Karlsson

O.H. Fridjonsson

P. Turner

G.O. Hreggvidson

J.K. Kristjansson

Olle Holst

Peter Schönheit

2007

Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324

Labes, A. ., & Schönheit, P. . (2007). Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324. Journal of Bacteriology, 189, 8901–8913. Abgerufen von http://oceanrep.geomar.de/7644/
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2007

Journal Article

Antje Labes

Peter Schönheit

Archaeoglobus fulgidus

starch degradation

cyclodextrin glucanotransferase

2005

Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria, have similar domain structures but different oligomeric states and activity profiles

Turner, P. ., Labes, A. ., Fridjonsson, O. H., Hreggvidson, G. O., Schönheit, P. ., Kristjansson, J. K., … Nordberg, K. E. (2005). Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria, have similar domain structures but different oligomeric states and activity profiles. Journal of Bioscience and Bioengineering, 100, 380–390. Abgerufen von http://oceanrep.geomar.de/168/

Abstract

In this paper, we present the expression and characterization of two novel enzymes from the \ensuremath\alpha-amylase family exhibiting cyclomaltodextrinase specificity. The nucleotide sequences encoding the enzymes were isolated from the genomic DNA of two thermophilic bacterial strains originating from Icelandic hot springs and belonging to the genera Anoxybacillus (AfCda13) and Laceyella (LsCda13). The genes were amplified using a consensus primer strategy utilizing two of the four conserved regions present in glycoside hydrolase family 13. No identifiable signal peptides were present in open reading frames encoding the enzymes, indicating an intracellular location of both enzymes, and their physiological function to be intracellular cyclodextrin degradation. The domain structures of both enzymes were also similar, including an N-terminal domain, the catalytic module composed of the A- and B-domains, and a C-terminal domain. Despite the similarity in domain composition, the two enzymes displayed differences in the oligomeric state with AfCda13 being a dimeric protein, whereas LsCda13 was monomeric. The two enzymes also displayed significantly different activity profiles, despite being active on the same range of substrates. It was shown that the enzyme displaying the highest activity on cyclodextrin was dimeric (AfCda13). Moreover, a fraction of the dimeric enzyme could be converted to a monomeric state in the presence of KCl and this fraction retained only 23\% of its activity on \ensuremath\alpha-cyclodextrin while its activity on starch was not significantly affected, indicating that the oligomeric state is an important factor for a high activity on cyclodextrin substrates.
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2005

Journal Article

Pernilla Turner

Antje Labes

Olafur Fridjonsson

Gudmundur Hreggvidson

Peter Schönheit

Jakob Kristjansson

Olle Holst

Karlsson Nordberg

cyclomaltodextrinase

glycoside hydrolase family 13

Anoxybacillus

Laceyella
Unusual starch degradation pathway in Archaeoglobus fulgidus strain 7324: Characterization of involved enzymes

Labes, A. ., & Schönheit, P. . (2005). Unusual starch degradation pathway in Archaeoglobus fulgidus strain 7324: Characterization of involved enzymes. Conference of association of general and applicated microbiology (VAAM). Abgerufen von http://oceanrep.geomar.de/2298/
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2005

Unpublished

Antje Labes

Peter Schönheit

Poster

2008

Novel members of glycoside hydrolase family 13 derived from environmental DNA

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2007

Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324

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2005

Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria, have similar domain structures but different oligomeric states and activity profiles

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Unusual starch degradation pathway in Archaeoglobus fulgidus strain 7324: Characterization of involved enzymes

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