TY - JOUR
KW - Microbiology
KW - Biology
KW - BIOSYNTHESIS GENE-CLUSTER
KW - COMPLETE GENOME SEQUENCE
KW - STREPTOMYCES SP
KW - BIOLOGICAL PROPERTIES
KW - POLYKETIDE SYNTHASE
KW - NATURAL-PRODUCTS
KW - PSEUDOCERATINA-CLAVATA
KW - ANTIMYCIN ANTIBIOTICS
KW - STRUCTURE ELUCIDATION
KW - CHONDRILLA-NUCULA
AU - Imke Schneemann
AU - Kerstin Nagel
AU - Inga Kajahn
AU - Antje Labes
AU - Jutta Wiese
AU - Johannes Imhoff
AB - Representatives of Actinobacteria were isolated from the marine  sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic exploration of biosynthetic genes, especially concerning the relationships of the abundance of biosynthesis gene fragments to the number and diversity of produced secondary metabolites. All strains were phylogenetically identified by 16S rRNA gene sequence analyses and were found to belong to the genera Actinoalloteichus,  Micrococcus, Micromonospora, Nocardiopsis, and Streptomyces. Secondary metabolite profiles of 46 actinobacterial strains were evaluated, 122 different substances were identified, and 88 so far unidentified compounds were detected. The extracts from most of the cultures showed biological activities. In addition, the presence of biosynthesis genes encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) in 30 strains was established. It was shown that strains in which either PKS or NRPS genes were identified produced a significantly higher number of metabolites and exhibited a larger number of unidentified, possibly new metabolites than other strains. Therefore, the presence of PKS and NRPS genes is a good indicator for the selection of strains to isolate new natural products.
BT - Applied and Environmental Microbiology
M1 - 11
N1 - WOS:000277871400034
N2 - Representatives of Actinobacteria were isolated from the marine  sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic exploration of biosynthetic genes, especially concerning the relationships of the abundance of biosynthesis gene fragments to the number and diversity of produced secondary metabolites. All strains were phylogenetically identified by 16S rRNA gene sequence analyses and were found to belong to the genera Actinoalloteichus,  Micrococcus, Micromonospora, Nocardiopsis, and Streptomyces. Secondary metabolite profiles of 46 actinobacterial strains were evaluated, 122 different substances were identified, and 88 so far unidentified compounds were detected. The extracts from most of the cultures showed biological activities. In addition, the presence of biosynthesis genes encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) in 30 strains was established. It was shown that strains in which either PKS or NRPS genes were identified produced a significantly higher number of metabolites and exhibited a larger number of unidentified, possibly new metabolites than other strains. Therefore, the presence of PKS and NRPS genes is a good indicator for the selection of strains to isolate new natural products.
PY - 2010
SP - 3702
EP - 3714
T2 - Applied and Environmental Microbiology
TI - Comprehensive Investigation of Marine Actinobacteria Associated with the Sponge Halichondria panicea
UR - http://oceanrep.geomar.de/8488/
VL - 76
ER -